Two epsilon glutathione S-transferase cDNAs from the common cutworm, Spodoptera litura: Characterization and developmental and induced expression by insecticides
Two epsilon glutathione S-transferase cDNAs from the common cutworm, Spodoptera litura: Characterization and developmental and induced expression by insecticides
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来自斜纹夜蛾、斜纹夜蛾的两种 ε 谷胱甘肽 S-转移酶 cDNA：特征、发育以及杀虫剂诱导表达
DOI：
10.1016/j.jinsphys.2009.08.017
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发表时间：
2009-12-01
影响因子：
2.2
通讯作者：
Zheng, Sichun
中科院分区：
文献类型：
--
作者：
Deng, Huimin;Huang, Yufen;Zheng, Sichun
两个斜纹夜蛾谷胱甘肽S-转移酶的cDNA（Slgste 2和Slgste 3），其中从中肠cDNA克隆，编码两个结构不同的蛋白质与预测的分子量分别为25和24 kDa。SIgste 2和Sigste 3在S.斜纹夜蛾基因组中没有内含子。Slgste 2和Slgste 3的转录本和蛋白质主要存在于5龄和6龄幼虫的中肠中。纯化的SlGSTE 2和SlGSTE 3重组蛋白对底物谷胱甘肽和1-氯-2，4-二硝基苯（CDNB）的表观Vmax相似。甲基氨基甲酸萘酯（西维因）、1，1，1-三氯-2，2-双（对氯苯基）乙烷（DDT）、溴氰菊酯、虫酰肼（RH 5992）和苏云金芽孢杆菌（Bt）均能上调Slgste 2的表达，而马拉硫磷对Slgste 2的表达无影响。西维因、Bt和DDT对Slgste 3的表达有轻微的上调作用，而RH 5992、马拉硫磷和溴氰菊酯对Slgste 3的表达没有影响。结果表明，Slgste 2和Slgste 3可能在棉铃虫对多种杀虫剂的解毒过程中发挥作用。斜纹。(C)2009爱思唯尔有限公司保留所有权利。
Two Spodoptera litura glutathione S-transferase cDNAs (Slgste2 and Slgste3) which were cloned from a midgut cDNA, encoded two structurally distinct proteins with a predicted molecular mass of 25 and 24 kDa, respectively. Slgste2 and Sigste3 were single-copy genes in the S. litura genome and there was no intron within the genes. The transcripts and proteins of Slgste2 and Slgste3 were predominately present in the midgut of the 5th and 6th instar larvae. The apparent Vmax of the purified SlGSTE2 and SlGSTE3 recombinant proteins towards the substrates glutathione and 1-chloro-2,4-dinitrobenezene (CDNB) were similar. Slgste2 expression was up-regulated by I-naphthyl methylcarbamate (carbaryl), 1,1,1-trichloro-2,2-bis-(p-chlorophenyl) ethane (DDT), deltamethrin, tebufenozide (RH5992) and Bacillus thuringiensis (Bt), but not affected by malathion. while Slgste3 expression was slightly up-regulated by carbaryl, Bt and DDT, but not affected by RH5992, malathion and deltamethrin. The results suggest that Slgste2 and Slgste3 may play roles in detoxifying various insecticides in S. litura. (C) 2009 Elsevier Ltd. All rights reserved.