A GLYCOLIPID ANTIGEN SPECIFIC TO MYCOBACTERIUM-PARATUBERCULOSIS - STRUCTURE AND ANTIGENICITY
A GLYCOLIPID ANTIGEN SPECIFIC TO MYCOBACTERIUM-PARATUBERCULOSIS - STRUCTURE AND ANTIGENICITY
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DOI：
10.1073/pnas.82.10.3068
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
BRENNAN, PJ
中科院分区：
文献类型：
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作者：
CAMPHAUSEN, RT;JONES, RL;BRENNAN, PJ
牛副结核病由于产奶量降低和受影响牛的寿命缩短而造成收入的巨大损失。M.副结核病（国家动物疾病中心菌株18和美国典型培养物保藏中心菌株19698）是约翰氏病（牛副结核病）的病原体，含有分离和表征的主要免疫反应性糖肽脂（极性GPL-I）。.**图形 **。糖脂抗原属于存在于其他分枝杆菌种属中的极性分枝杆菌苷C糖肽脂家族。远端的2，3-二-O-甲基-α- L-岩藻吡喃糖基-（1 →3）-α- L-吡喃鼠李糖苷单元是M.副结核病糖脂在M.通过TLC [薄层色谱法]检测副结核杆菌，其存在可用作感染原的特征性标志物。极性糖肽脂在ELISA [酶联免疫吸附试验]中对M.副结核杆菌菌株18，表明其基本的免疫原性。
Bovine paratuberculosis causes large losses in revenue due to lower milk production and shorter life for affected cattle. M. paratuberculosis (National Animal Disease Center strain 18 and American Type Culture Collection strain 19698), the causative agent of Johne disease (bovine paratuberculosis), contains a major immunoreactive glycopeptidolipid (polar GPL-I) that was isolated and characterized. .**GRAPHIC**. The glycolipid antigen belongs to the polar mycoside C glycopeptidolipid family present in other mycobacterial species. The distal 2,3-di-O-methyl-.alpha.-L-fucopyranosyl-(1 .fwdarw. 3)-.alpha.-L-rhamnopyranoside unit, the obvious antigen determinant, appears to be characteristic of M. paratuberculosis. The glycolipid can be recognized readily in isolates of M. paratuberculosis by TLC [thin layer chromatography] and its presence may be used as a characteristic marker of the infectious agent. The polar glycopeptidolipid was highly reactive in ELISA [enzyme-linked immunosorbent assay] against serum from an animal hyperimmunized with M. paratuberculosis strain 18, indicating its basic immunogenicity.