Mouse Eya genes are expressed during limb tendon development and encode a transcriptional activation function
Mouse Eya genes are expressed during limb tendon development and encode a transcriptional activation function
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DOI：
10.1073/pnas.94.22.11974
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发表时间：
1997-10-28
影响因子：
11.1
通讯作者：
Maas, RL
中科院分区：
文献类型：
--
作者：
Xu, PX;Cheng, J;Maas, RL
脊椎动物肢腱来源于侧板中胚层的结缔细胞，导致脊椎动物肢腱形成的一些发育步骤先前已被确定;然而，在胚胎发生过程中负责腱的形成和维持的分子机制在很大程度上是未知的。果蝇的眼缺失（eya）基因编码一种新的分子功能未知的核蛋白，在这里，我们发现Eya 1和Eya 2，果蝇Eya的两个小鼠同源物，最初在肢体发育过程中在结缔组织前体细胞中表达，后来在肢体发育中，Eya 1和Eya 2表达与形成不同肢体肌腱组的细胞凝聚有关，Eya 1表达主要限于屈肌腱，而Eya 2在肢体的趾部的伸肌腱和韧带中表达。这些数据表明，Eya基因参与了肢体远端肌腱的形成。为了研究Eya基因产物的分子功能，我们分析了高度分化的PST是否Eya 1 -3的富含脯氨酸-丝氨酸-苏氨酸的N端区域作为转录激活结构域发挥功能，我们的结果表明Ega基因产物可以作为转录激活因子，并且它们支持这种分子功能在结缔组织形成中的作用。
Vertebrate limb tendons are derived from connective cells of the lateral plate mesoderm, Some of the developmental steps leading to the formation of vertebrate limb tendons have been previously identified; however, the molecular mechanisms responsible for tendinous patterning and maintenance during embryogenesis are largely unknown, The eyes absent (eya) gene of Drosophila encodes a novel nuclear protein of unknown molecular function, Here we show that Eya1 and Eya2, two mouse homologues of Drosophila eya, are expressed initially during limb development in connective tissue precursor cells, Later in limb development, Eya1 and Eya2 expression is associated with cell condensations that form different sets of limb tendons, Eya1 expression is largely restricted to flexor tendons, while Eya2 is expressed in the extensor tendons and ligaments of the phalangeal elements of the limb. These data suggest that Eya genes participate in the patterning of the distal tendons of the limb, To investigate the molecular functions of the Eya gene products, we have analyzed whether the highly divergent PST (proline-serine-threonine)-rich N-terminal regions of Eya1-3 function as transactivation domains, Our results demonstrate that Ega gene products can act as transcriptional activators, and they support a role for this molecular function in connective tissue patterning.