Quantitation of in vitro opsonic activity of human antibody induced by a vaccine consisting of the type III-specific polysaccharide of group B streptococcus
Quantitation of in vitro opsonic activity of human antibody induced by a vaccine consisting of the type III-specific polysaccharide of group B streptococcus
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由 B 族链球菌 III 型特异性多糖组成的疫苗诱导的人抗体体外调理活性的定量
DOI：
10.1128/iai.39.3.1155-1160.1983
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发表时间：
1983
影响因子：
3.1
通讯作者：
R. M. Swenson
中科院分区：
文献类型：
--
作者：
B. J. de Cueninck;T. Eisenstein;T. McIntosh;G. Shockman;R. M. Swenson
由未降解和高度纯化的B群链球菌胞外iii型特异性多糖组成的疫苗诱导的人抗体，显示出提高吞噬细胞介导的对同源型细菌的杀伤率。其杀菌作用是由iii型特异性抗体介导的，具有补体依赖性。开发了一种允许定量测定“声速活性”的测定方法。在这个实验中，cfu的损失以恒定的速率发生，并且速率常数被用作抗血清的声速活性的测量。iii型特异性抗体浓度（40 ~ 500 ng/ml）与杀伤速率常数呈线性关系。当检测免疫血清组时，一些血清反应异常，介导率明显高于或低于基于其抗体含量的预期。由于免疫血清中iii型特异性抗体几乎全部在免疫球蛋白G类中发现，我们假设特异性抗体在免疫球蛋白G亚类分布的差异可能是这种差异的来源。
Human antibody, induced by a vaccine consisting of undegraded and highly purified extracellular type III-specific polysaccharide of group B streptococcus, was shown to increase the rate of phagocyte-mediated killing of bacteria of the homologous type. The bactericidal effect was mediated by type III-specific antibody and was complement dependent. An assay which permitted quantitation of "opsonic activity" was developed. In this assay, loss of CFUs occurred at a constant rate, and the rate constant was used as a measure of opsonic activity of antisera. A linear relationship between type III-specific antibody concentration (40 to 500 ng/ml) and the rate constant of killing was observed. When sets of immune sera were tested, some sera reacted anomalously, mediating significantly higher or lower rates than expected on the basis of their antibody content. Since type III-specific antibody in immune sera was found almost exclusively in the immunoglobulin G class, we hypothesize that differences in immunoglobulin G subclass distribution of specific antibody may have been the source of this variation.