Transcription of human neuronal nitric oxide synthase mRNAs derived from different first exons is partly controlled by exon 1-specific promoter sequences
Transcription of human neuronal nitric oxide synthase mRNAs derived from different first exons is partly controlled by exon 1-specific promoter sequences
复制标题
DOI：
10.1016/j.ygeno.2005.11.013
复制
发表时间：
2006-04-01
期刊：
影响因子：
4.4
通讯作者：
Förstermann, U
中科院分区：
文献类型：
--
作者：
Bros, M;Boissel, JP;Förstermann, U
人神经元型一氧化氮合酶（NOSI）基因是一个广泛剪接的基因。共鉴定出12种NOSI mRNA。它们的5'末端不同，并且衍生自12个不同的第一外显子（称为外显子Ia至II）。鉴定了其NOSI第一外显子表达模式代表人脑、皮肤和骨骼肌的各种细胞系。这些细胞包括A673神经上皮瘤细胞、SK-N-MC神经母细胞瘤细胞、HaCaT角质形成细胞样细胞和C2 C12肌细胞样细胞。在这些细胞系中，发现优先表达的外显子I变体与其同源5'侧翼序列的启动子活性之间存在相关性。这些数据表明，NOSI mRNA的不同外显子I相关剪接变体的表达直接（至少部分）受相关5'侧翼序列控制。(c)2005年爱思唯尔公司All rights reserved.
The human neuronal nitric oxide synthase (NOSI) gene is subject to extensive splicing. A total of 12 NOSI mRNA species have been identified. They differ in their 5' ends and are derived from 12 different first exons (termed exons Ia to II). Various cell lines whose NOSI first exon expression patterns were representative of human brain, skin, and skeletal muscle were identified. These included A673 neuroepithelioma cells, SK-N-MC neuroblastoma cells, HaCaT keratinocyte-like cells, and C2C12 myocyte-like cells. In these cell lines, correlations were found between the exon I variants preferentially expressed and the promoter activities of their cognate 5' flanking sequences. These data demonstrate that expression of the different exon I-related splice variants of NOSI mRNA is controlled directly (at least in part) by the associated 5' flanking sequences. (c) 2005 Elsevier Inc. All rights reserved.