Chemico-enzymatic synthesis of a new fluorescent-labeled DNA by PCR with a thymidine nucleotide analogue bearing an acridone derivative.
Chemico-enzymatic synthesis of a new fluorescent-labeled DNA by PCR with a thymidine nucleotide analogue bearing an acridone derivative.
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DOI：
10.1016/j.bmcl.2006.10.072
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发表时间：
2007-02
影响因子：
2.7
通讯作者：
Atsushi Shoji;Tomoya Hasegawa;M. Kuwahara;H. Ozaki;H. Sawai
中科院分区：
文献类型：
--
作者：
Atsushi Shoji;Tomoya Hasegawa;M. Kuwahara;H. Ozaki;H. Sawai
制备了含有吖啶酮的胸腺嘧啶类似物的三磷酸，并使用KOD Dash DNA聚合酶作为PCR的底物，形成了一种新的荧光标记DNA，可作为DNA探针。
Triphosphate of a thymidine analogue bearing acridone was prepared and incorporated as a substrate for PCR using KOD Dash DNA polymerase forming a new fluorescent-labeled DNA that is useful as a DNA probe.