THE INDIVIDUAL ANTIGENIC SPECIFICITY OF ANTIBODIES TO STREPTOCOCCAL CARBOHYDRATES
THE INDIVIDUAL ANTIGENIC SPECIFICITY OF ANTIBODIES TO STREPTOCOCCAL CARBOHYDRATES
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链球菌碳水化合物抗体的个体抗原特异性
DOI：
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发表时间：
1968
影响因子：
15.3
通讯作者：
R. Krause
中科院分区：
文献类型：
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作者：
D. Braun;R. Krause
尽管在用链球菌疫苗免疫的家兔中，对碳水化合物组具有特异性的单一免疫学上均匀的抗体组分可能构成大部分的γ-球蛋白，但情况并非总是如此。并非罕见，电泳可揭示多种抗体成分。然而，通过各种制备方法从单一抗血清中分离出至少两种对碳水化合物具有相似反应性的抗体组分是可行的，这两种抗体组分都是单分散的。当通过圆盘电泳检查时，来自此类抗体的轻链显示限制性模式。已经检查了链球菌碳水化合物的抗体的个体抗原特异性。用分离的C组和A组变异体抗体免疫山羊。这些抗体的个体抗原特异性通过用混合的正常兔血清的Fr II吸收山羊抗血清而产生。山羊抗抗血清与Fr II的额外吸收减少，但没有消除同源抗体的反应性。用纯化的链球菌抗体的木瓜蛋白酶片段进行的免疫电泳研究将特异性定位于Fab片段。特异性不限于抗体的分离轻链。
Although a single electrophoretically uniform antibody component with specificity for the group carbohydrate may comprise the bulk of the γ-globulin in rabbits immunized with streptococcal vaccines, this is not always the case. Not infrequently, electrophoresis may reveal multiple antibody components. Nevertheless, it has been feasible by various preparative procedures to isolate from a single antiserum at least two antibody components with similar reactivity for the carbohydrate both of which are electrophoretically monodisperse. Light chains from such antibodies reveal a restricted pattern when examined by disc electrophoresis. Antibodies to streptococcal carbohydrates have been examined for their individual antigenic specificity. Goats were immunized with isolated Group C and Group A-variant antibodies raised in rabbits. Individual antigenic specificity of these antibodies was brought out by absorption of the goat anti-antiserum with Fr II of pooled normal rabbit sera. Additional absorption of the goat anti-antisera with Fr II diminished but did not eliminate the reactivity for the homologous antibody. Immunoelectrophoretic studies with papain fragments of purified streptococcal antibodies localized the specificity to the Fab fragment. Specificity was not confined to the isolated light chains of the antibody.