Lactic acid decontamination of fresh pork carcasses: a pilot plant study.
Lactic acid decontamination of fresh pork carcasses: a pilot plant study.
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新鲜猪肉胴体的乳酸净化：一项中试工厂研究。
DOI：
10.1016/0168-1605(94)00039-9
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
J. H. J. Huis in ’t Veld
中科院分区：
文献类型：
--
作者：
P. van Netten;D. Mossel;J. H. J. Huis in ’t Veld
在屠宰场对人工污染粪便中鼠伤寒沙门氏菌的猪肉进行乳酸去污(LAD)。鼠伤寒沙门氏菌的表面污染范围为1-2log10cfu cm2。在进行冷热LAD之前，让接种物在肉表面黏附20min。冷处理组60只S用2%(pH 2.3)或5%(pH 1.9)乳酸(LA)处理，热处理组暴露次数分别为30、60、90和120次S，喷嘴温度分别为11℃和55℃，处理后的肉表面温度分别为16~18℃和36~38℃。用2%和5%LA冷处理60头S，可从接种约1log10cfu cm2的猪肉屠体中消灭鼠伤寒沙门氏菌，但不能消除接种约2log10cfu cm2的猪肉中的鼠伤寒沙门氏菌。然而，这可以通过热2%和5%的LA喷洒60-120 S来实现。同时，用这些热LA溶液对至少30个S暴露，从接种约1log10cfu cm~2的身体上一致地杀灭鼠伤寒沙门氏菌。清洗对减少污染的作用微乎其微。2%或5%乳酸处理使S肉的感官品质发生了不可接受的劣变。在喷雾溶液中加入烟酸和抗坏血酸作为颜色稳定剂，当使用2%LA时，这些变化会减少到刚刚可以接受的水平。
Lactic acid decontamination (LAD) was carried out in an abattoir on pork carcasses, artificially contaminated with Salmonella typhimurium in faeces suspensions. The surface contamination with S. typhimurium ranged from 1–2 log10cfu cm2. Before cold and hot LAD was undertaken, the inoculum was allowed to adhere to the meat surface for 20 min. Cold LAD consisted of treatment for 60 s with 2% (pH 2.3) or 5% (pH 1.9) lactic acid (LA); for hot LAD the exposure times were 30, 60, 90 and 120 s. The spray nozzle temperatures were 11 °C and 55 °C, and that of the treated meat surface 16–18 °C and 36–38 °C, respectively. Treatment with cold 2% and 5% LA for 60 s eliminated S. typhimurium from pork carcasses inoculated with ca. 1 log10cfu cm2, but not from those inoculated at ca. 2 log10cfu cm2. However, this could be achieved by hot 2% and 5% LA sprayed for 60–120 s. Also exposures of at least 30 s using these hot LA solutions eliminated S. typhimurium consistently from carcasses inoculated with ca. 1 log10cfu cm2. Rinsing-off contributed only marginally to contamination reduction. Application of 2% or 5% LA for 120 s led to an unacceptable deterioration of the organoleptic qualities of the meat. Addition of nicotinic and ascorbic acid as colour stabilisers to the spraying solutions reduced these changes to just acceptable levels when 2% LA was used.