Use of mu-conotoxin GIIIA for the study of synaptic transmission at the frog neuromuscular junction.
Use of mu-conotoxin GIIIA for the study of synaptic transmission at the frog neuromuscular junction.
复制标题
使用 mu-芋螺毒素 GIIIA 研究青蛙神经肌肉接头处的突触传递。
DOI：
10.1016/0304-3940(93)90745-7
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发表时间：
1993
影响因子：
2.5
通讯作者：
Zengel,JE
中科院分区：
文献类型：
--
作者：
Sosa,MA;Zengel,JE
我们研究了合成的μ-芋螺毒素GIIIA（一种肌肉NA通道的选择性阻断剂）对青蛙缝匠肌神经-肌肉制备物上突触传递的各种参数的影响。我们发现5 μM μ-芋螺毒素持续阻断肌肉动作电位，但对神经动作电位没有影响。毒素也没有影响的幅度或频率的微型终板电位（MEPPs），终板电位（EPPs）的幅度或时间过程中，或刺激诱导的变化EPP幅度。合成的μ-芋螺毒素GIIIA对递质释放没有影响，这使得该毒素成为研究正常释放水平条件下神经肌肉传递的宝贵工具。
We have looked at the effect of synthetic μ-conotoxin GIIIA, a selective blocker of muscle NA channels, on various parameters of synaptic transmission at the frog sartorius nerve-muscle preparation. We found that 5 μM μ-conotoxin consistently blocked muscle action potentials, but had no effect on nerve action potentials. The toxin also had no effect on the amplitude or frequency of miniature endplate potentials (MEPPs), on the amplitude or time course of endplate potentials (EPPs), or on stimulation-induced changes in EPP amplitude. The lack of an effect of synthetic μ-conotoxin GIIIA on transmitter release makes this toxin an invaluable tool in the study of neuromuscular transmission under conditions of normal levels of release.