Magnetic resonance imaging of nude mice with heterotransplanted high-grade squamous cell carcinomas - Use of a low-loaded, covalently bound Gd-Hsa conjugate as contrast agent with high tumor affinity
Magnetic resonance imaging of nude mice with heterotransplanted high-grade squamous cell carcinomas - Use of a low-loaded, covalently bound Gd-Hsa conjugate as contrast agent with high tumor affinity
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DOI：
10.1097/00004424-200204000-00004
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发表时间：
2002-04-01
影响因子：
6.7
通讯作者：
Delorme, S
中科院分区：
文献类型：
--
作者：
Kiessling, F;Fink, C;Delorme, S
基本原理。恶性肿瘤通常表现为血浆蛋白摄取和代谢增加，尤其是白蛋白。目的：确定低负荷Gd-白蛋白的积聚是否改善了恶性肿瘤的磁共振成像。方法：对12只裸鼠的异种移植鳞癌进行研究。观察注射Gd-白蛋白144h后肿瘤、血液、肝脏、肾脏和肌肉组织的MRI信号强度。9只裸鼠同时注射Gd和荧光素标记的白蛋白后，MRI结果与组织学结果相关。结果：虽然肝脏和肾脏的信号强度在30分钟内达到最大值，但肿瘤在注射后24小时内表现出51%的延迟增长。组织学和荧光评估显示白蛋白主要分布在肿瘤的间质和坏死中。结论：Gd-白蛋白在SCC移植中有效积聚。低负荷Gd-白蛋白的MRI可能为识别对胞质药物标记白蛋白治疗敏感的肿瘤提供相关机会。
RATIONALE. Malignant tumors often show an increased uptake and metabolism of plasma proteins, especially albumin.OBJECTIVES.Determine whether the accumulation of low loaded Gd-albumin improves visualization of malignant tumors by MRI.METHODS. Twelve nude mice with heterotransplanted squamous cell carcinomas were studied. The signal intensity of tumor, blood, liver, kidney and muscle tissue was studied in MR images after application of Gd-albumin during a period of 144 hours. MRI results were histologically correlated after simultaneously injection of Gd- and fluorescein-labeled albumins In 9 nude mice.RESULTS. Although liver and kidney had a maximum increase In signal intensity within 30 minutes, tumors showed a delayed 51% increase in the 24 hours after application. Histologic and fluorescence evaluation demonstrated albumin localization in tumors predominantly in stroma and necroses.CONCLUSIONS. Gd-albumin is efficiently accumulated in SCC transplants. MRI with low loaded Gd-albumin may offer relevant opportunities for recognizing tumors sensitive to a therapy with cyostic drug-labeled albumins.