In vitro evidence for effects of magnesium supplementation on quinolone-treated horse and dog chondrocytes
In vitro evidence for effects of magnesium supplementation on quinolone-treated horse and dog chondrocytes
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DOI：
10.1354/vp.38-2-143
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发表时间：
2001-03-01
影响因子：
2.4
通讯作者：
Gabler, C
中科院分区：
文献类型：
--
作者：
Egerbacher, M;Wolfesberger, B;Gabler, C
喹诺酮类药物和镁缺乏症在幼年动物的关节软骨中引起类似的病变。在喹诺酮类药物存在下和无镁培养基中培养的软骨细胞显示细胞骨架的严重改变和粘附于培养皿的能力下降。我们研究了镁离子补充是否可以防止喹诺酮介导的体外软骨细胞的影响。用环丙沙星（80和160 μ g/ml）和恩诺沙星（100和150 μ g/ml）处理在Dulbecco改良Eagle培养基/HAM F-12培养基中培养的软骨细胞。以0.0612 mg/ml（MgCl）和0.0488 mg/ml（MgSO 4）的浓度或三倍剂量加入Mg 2+。此外，将细胞在无Mg培养基中培养，并相应地用Mg 2+补充物处理。培养5天后，测定每毫升贴壁细胞的数量。在培养期间，喹诺酮处理组的软骨细胞数量减少至对照组的12-36%。随着Mg ~（2+）的补充，贴壁细胞的数量增加到对照细胞的40-70%。三倍剂量的Mg 2+导致比单剂量更好的结果。用Ki 67（克隆MIB 5）免疫组织化学染色检测的细胞增殖以浓度依赖性方式（50、100和150 μ g/ml）从对照组的70%降低到恩诺沙星处理组的55%、48%和30%。加入Mg ~（2+）并不增加细胞增殖率。这些结果表明，很大一部分喹诺酮类药物诱导的损伤是由于镁复合物的形成，因为Mg 2+补充剂能够降低体外效应。然而，喹诺酮对细胞增殖的影响似乎是一个独立的过程，不受镁补充的影响。
Quinolones and magnesium deficiency cause similar lesions in joint cartilage of young animals. Chondrocytes cultivated in the presence of quinolones and in Mg-free medium show severe alterations in cytoskeleton and decreased ability to adhere to the culture dish. We investigated whether Mg2+ supplementation can prevent quinolone-mediated effects on chondrocytes in vitro. Chondrocytes cultivated in Dulbecco's modified Eagle's medium/HAM's F-12 medium were treated with ciprofloxacin (80 and 160 mug/ml) and enrofloxacin (100 and 150 mug/ml). Mg2+ was added at a concentration of 0.0612 mg/ml (MgCl) and 0.0488 mg/ml (MgSO4) or a triple dose. In addition, cells were cultivated in Mg-free medium and accordingly treated with Mg2+ supplementation. After 5 days in culture, the number of adherent cells per milliliter was determined. The number of chondrocytes in quinolone-treated groups decreased to 12-36% that of the control group within the culture period. With Mg2+ supplementation, the number of attached cells increased to 40-70% that of control cells. The threefold dose of Mg2+ led to better results than did the single dose. Cell proliferation tested by immunohistochemical staining with Ki67 (clone MIB5) decreased from 70% in control groups to 55%, 48%, and 30% in enrofloxacin-treated groups in a concentration dependent manner (50, 100, and 150 mug/ml). Addition of Mg2+ did not increase the rate of cell proliferation. These results suggest that a great part of quinolone-induced damage is due to magnesium complex formation, as Mg2+ supplementation is able to reduce the effects in vitro. However, quinolone effects on cell proliferation seem to be an independent process that is not influenced by magnesium supplementation.