Identification and Characterization of Genes Required for Utilization of Desferri-Ferrichrome and Aerobactin in Vibrio parahaemolyticus
Identification and Characterization of Genes Required for Utilization of Desferri-Ferrichrome and Aerobactin in Vibrio parahaemolyticus
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DOI：
10.1248/bpb.32.359
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发表时间：
2009-03-01
影响因子：
2
通讯作者：
Yamamoto, Shigeo
中科院分区：
文献类型：
--
作者：
Funahashi, Tatsuya;Tanabe, Tomotaka;Yamamoto, Shigeo
在我们的调查过程中的铁收购系统的副溶血性弧菌，一个致病因子的海鲜相关的胃肠炎，我们发现，这种物种利用desferri-ferrichrome生长作为一种异源铁载体（铁载体产生的其他细菌和真菌）在铁限制条件下。铁抑制性外膜蛋白的N-末端氨基酸序列分析，然后搜索该物种的报告基因组序列，确定了形成操纵子的四个相关基因（称为fhuACDB）。fhuA和fhuD基因的缺失分析表明，最上游的基因fhuA和三个下游基因fhuCDB编码的铁色素受体和ATP结合盒转运组件，分别。此外，人们发现fhuCDB基因也是运输铁需氧肌动蛋白所必需的，铁需氧肌动蛋白可以在铁限制条件下恢复该物种的生长。
During the course of our investigation on the iron acquisition systems in Vibrio parahaemolyticus, a causative agent of seafood-related gastroenteritis, we found that this species utilizes desferri-ferrichrome for growth as a heterologous siderophore (a siderophore produced by other bacteria and fungi) under iron-limiting conditions. N-Terminal amino acid sequence analysis of the iron-repressible outer membrane proteins followed by searches of the reported genomic sequences of this species identified four relevant genes (called fhuACDB) forming an operon. Deletion analysis of the fhuA and fhuD genes indicated that the most upstream gene fhuA and the three downstream genes fhuCDB encode the ferrichrome receptor and the ATP-binding cassette transport components, respectively. Moreover, it was found that the fhuCDB genes are also required for transport of ferric aerobactin which restores growth of this species under iron-limiting conditions.