Human osteoblast-like cells respond to mechanical strain with increased bone matrix protein production independent of hormonal regulation.
Human osteoblast-like cells respond to mechanical strain with increased bone matrix protein production independent of hormonal regulation.
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DOI：
10.1210/endo.136.2.7530647
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发表时间：
1995-02
期刊：
影响因子：
4.8
通讯作者：
L. Harter;K. Hruska;R. Duncan
中科院分区：
文献类型：
--
作者：
L. Harter;K. Hruska;R. Duncan
骨肉瘤细胞系暴露于慢性间歇性应变会增加机械敏感阳离子（SA-cat）通道的活性。机械传导对成骨细胞功能的影响尚未得到充分研究。我们分析了人成骨细胞样骨肉瘤细胞 OHS-4 中骨基质蛋白响应慢性间歇性机械应变的表达和产生。 OHS-4 细胞表现出 I 型胶原蛋白的产生、1,25-二羟基维生素 D 诱导的骨钙素和细胞外基质的矿化。基质蛋白信息水平是通过从暴露于慢性间歇性菌株1-4天的细胞中分离出的总RNA确定的。 I 型胶原蛋白的 Northern 分析表明，48 小时后，应变增加了胶原蛋白信息。 I 型胶原蛋白的免疫荧光标记表明，机械应变也可增强分泌。在缺乏维生素 D 的情况下，通过施加机械负荷，骨桥蛋白信息水平会增加数倍，并且两种刺激共同产生相加效应。骨钙素分泌也随着循环应变而增加。在未经维生素 D 处理的对照细胞中未检测到骨钙素水平。然而，诱导负荷 4 天后，在培养基中观察到显着水平的骨钙素。在存在维生素 D 的情况下，应变细胞培养基中的骨钙素水平比非应变对照高 4 倍。我们得出的结论是，成骨细胞样细胞的机械应变足以通过机械转导增加基质蛋白的转录和分泌，而无需激素诱导。
Exposure of osteosarcoma cell lines to chronic intermittent strain increases the activity of mechano-sensitive cation (SA-cat) channels. The impact of mechano-transduction on osteoblast function has not been well studied. We analyzed the expression and production of bone matrix proteins in human osteoblast-like osteosarcoma cells, OHS-4, in response to chronic intermittent mechanical strain. The OHS-4 cells exhibit type I collagen production, 1,25-Dihydroxyvitamin D-inducible osteocalcin, and mineralization of the extracellular matrix. The matrix protein message level was determined from total RNA isolated from cells exposed to 1-4 days of chronic intermittent strain. Northern analysis for type I collagen indicated that strain increased collagen message after 48 h. Immunofluorescent labeling of type I collagen demonstrated that secretion was also enhanced with mechanical strain. Osteopontin message levels were increased several-fold by the application of mechanical load in the absence of vitamin D, and the two stimuli together produced an additive effect. Osteocalcin secretion was also increased with cyclic strain. Osteocalcin levels were not detectable in vitamin D-untreated control cells. However, after 4 days of induced load, significant levels of osteocalcin were observed in the medium. With vitamin D present, osteocalcin levels were 4 times higher in the medium of strained cells compared to nonstrained controls. We conclude that mechanical strain of osteoblast-like cells is sufficient to increase the transcription and secretion of matrix proteins via mechano-transduction without hormonal induction.