Determination of Organic Acids in Honey by Liquid Chromatography with Tandem Mass Spectrometry
Determination of Organic Acids in Honey by Liquid Chromatography with Tandem Mass Spectrometry
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DOI：
10.1007/s12161-020-01845-w
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发表时间：
2020-08-29
影响因子：
2.9
通讯作者：
Nakamura, Yasunori
中科院分区：
文献类型：
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作者：
Suto, Momoka;Kawashima, Hiroto;Nakamura, Yasunori
蜂蜜中的有机酸目前通过酶法、毛细管区带电泳和色谱法进行定量;然而，这些方法分析时间长，灵敏度和选择性低。在这里，我们开发了一个简单，快速的液相色谱-串联质谱（LC-MS/MS）为基础的方法测定有机酸：葡萄糖酸，酒石酸，苹果酸，柠檬酸，琥珀酸蜂蜜。首先，我们评估了线性，基质效应，检测限，定量限，和我们的方法的重复性;有机酸的检测限范围为0.005至0.70 mg/kg。在本研究中，它的灵敏度比高效液相色谱法高8至31倍。然后，我们用我们的方法来确定葡萄糖酸，酒石酸，苹果酸，柠檬酸和琥珀酸的浓度在25个蜂蜜样品。葡萄糖酸占样品中总有机酸的64.6 - 99.8%。通过对各组分的相关性和统计分析，认为有机酸是由葡萄糖氧化酶等工蜂酶和TCA循环产生的。所开发的方法具有高灵敏度和高选择性，将能够分析更复杂和低浓度的组分。
Organic acids in honeys are currently quantified by enzymatic, capillary zone electrophoresis, and chromatographic methods; however, these methods have long analysis times and low sensitivities and selectivities. Here, we developed a simple, rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for the determination of organic acids: gluconic acid, tartaric acid, malic acid, citric acid, and succinic acid in honey. First, we evaluated the linearity, matrix effects, detection limits, quantification limits, and repeatability of our method; the limit of detection for organic acids ranged from 0.005 to 0.70 mg/kg. In this study, it was 8 to 31 times higher sensitive than the high-performance liquid chromatography method. We then used our method to determine the concentrations of gluconic acid, tartaric acid, malic acid, citric acid, and succinic acid in 25 honey samples. Gluconic acid was found to account for 64.6 to 99.8% of the total organic acids in the samples. From the correlation and statistical analysis of each component, it is considered that the organic acid is produced by worker bee enzymes such as glucose oxidase and the TCA cycle. The developed method, which has high sensitivity and selectivity, will enable the analysis of more complex and low-concentration components.