Observation of Inhomogeneity in the Lipid Composition of Individual Nanoscale Liposomes
Observation of Inhomogeneity in the Lipid Composition of Individual Nanoscale Liposomes
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DOI：
10.1021/ja203984j
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发表时间：
2011-07-20
影响因子：
15
通讯作者：
Stamou, Dimitrios
中科院分区：
文献类型：
--
作者：
Larsen, Jannik;Hatzakis, Nikos S.;Stamou, Dimitrios
脂质体或囊泡作为生物膜模型和药物递送载体已被广泛研究。通常假定同一制剂中的所有脂质体都是相同的。在这里，通过采用对荧光标记的脂质，我们证明了高达10倍的变化，在个别脂质体的相对脂质组成的直径在50 nm和15 μ m之间。由于脂质体的物理化学性质与其组成直接相关，因此组成不均匀性的直接后果是整体中单个脂质体性质的多分散性。
Liposomes, or vesicles, have been studied extensively both as models of biological membranes and as drug delivery vehicles. Typically it is assumed that all liposomes within the same preparation are identical. Here by employing pairs of fluorescently labeled lipids we demonstrated an up to 10-fold variation in the relative lipid composition of individual liposomes with diameters between 50 nm and 15 mu m. Since the physicochemical properties of liposomes are directly linked to their composition, a direct consequence of compositional inhomogeneities is a polydispersity in the properties of the individual liposomes in an ensemble.