Live imaging of lymphatic development in the zebrafish
Live imaging of lymphatic development in the zebrafish
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DOI：
10.1038/nm1427
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发表时间：
2006-06-01
期刊：
影响因子：
82.9
通讯作者：
Weinstein, Brant M.
中科院分区：
文献类型：
--
作者：
Yaniv, Karina;Isogai, Sumio;Weinstein, Brant M.
近年来，淋巴系统因其在正常和病理过程中的重要作用而成为人们非常感兴趣的课题。然而，在了解淋巴系统的起源和早期发展方面的进展一直受到在体内观察淋巴细胞以及在目前可用的模式生物中对淋巴系统进行明确的遗传和实验操作的困难的阻碍。在这里，我们展示了光学清晰的发育中的斑马鱼为成像和研究淋巴发育提供了一个有用的模型，它的淋巴系统与其他脊椎动物的淋巴管有许多共同的形态学、分子和功能特征。利用转基因斑马鱼的双光子延时成像技术，我们追踪了融入淋巴内皮的单个细胞的迁移和谱系。我们的研究结果表明，胸导管的淋巴内皮细胞通过一种新的和意想不到的途径从原始静脉产生。
The lymphatic system has become the subject of great interest in recent years because of its important role in normal and pathological processes. Progress in understanding the origins and early development of this system, however, has been hampered by difficulties in observing lymphatic cells in vivo and in performing defined genetic and experimental manipulation of the lymphatic system in currently available model organisms. Here, we show that the optically clear developing zebrafish provides a useful model for imaging and studying lymphatic development, with a lymphatic system that shares many of the morphological, molecular and functional characteristics of the lymphatic vessels found in other vertebrates. Using two-photon time-lapse imaging of transgenic zebrafish, we trace the migration and lineage of individual cells incorporating into the lymphatic endothelium. Our results show lymphatic endothelial cells of the thoracic duct arise from primitive veins through a novel and unexpected pathway.