Distribution of activated neurons in the rabbit brain following a volume load
Distribution of activated neurons in the rabbit brain following a volume load
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DOI：
10.1016/s0306-4522(97)00232-7
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发表时间：
1997-12-01
期刊：
影响因子：
3.3
通讯作者：
McGrath, BP
中科院分区：
文献类型：
--
作者：
Badoer, E;McKinlay, D;McGrath, BP
免疫组织化学检测的蛋白质，Fos，被用来确定在脑中的神经元激活后的容量负荷。将血浆扩张剂海脉素和6%右旋糖酐静脉输注到清醒的兔子体内60分钟。与对照动物相比，这两种刺激均显着增加右心房压，但对血压没有影响。仅使用右旋糖酐时心率显著升高。海脉素扩容还使肾交感神经活性较输注前静息水平降低约50%。输注开始后90分钟，将家兔灌注固定，并检查Fos阳性细胞核的分布。海脉素输注后，终板、小细胞室旁核的血管器中Fos阳性细胞核的数量显著增加，孤束核和延髓腹外侧核的特定喙尾侧水平也显著增加。葡聚糖后，在延髓中观察到类似的效果，但Fos阳性细胞核在前脑中没有显着高于对照。后海脉素或葡聚糖领域，如视上核，大细胞室旁核，床核的纹终末，斜角带的布罗卡和杏仁核要么不产生Fos或不一致从对照组不同的结果表明，特定的大脑区域，这是已知的是重要的心血管控制，激活的体积负荷。这些区域可能在由特定刺激引发的反射反应中发挥重要作用。(C)1997年IBRO。出版社：Elsevier Science Ltd
Immunohistochemical detection of the protein, Fos, was used to identify neurons in the brain activated following a volume load. The plasma expanders, Haemaccel and 6% dextran, were infused intravenously in conscious rabbits for 60 min. Compared to control animals both stimuli significantly increased right atrial pressure but had no effect on blood pressure. Heart rate was significantly elevated with dextran only. Volume expansion with Haemaccel also reduced renal sympathetic nerve activity by about 50% from the pre-infusion resting level. Ninety minutes after the start of the infusion, the rabbits were perfusion fixed and the distribution of Fos-positive cell nuclei was examined. Following Haemaccel infusion there were significant increases in the number of Fos-positive cell nuclei in the organum vasculosum of the lamina terminalis, parvocellular paraventricular nucleus and in specific rostrocaudal levels of the nucleus tractus solitarius and ventrolateral medulla. Following dextran similar effects were observed in the medulla but Fos-positive cell nuclei were not significantly elevated above controls in the forebrain. After Haemaccel or dextran areas such as the supraoptic nucleus, the magnocellular paraventricular nucleus, the bed nucleus of the stria terminalis, diagonal band of Broca and amygdala either did not produce Fos or were not consistently different from the control group.The results suggest that specific brain regions, that are known to be important in cardiovascular control, are activated by a volume load. These areas are likely to play an important role in the reflex responses initiated by that particular stimulus. (C) 1997 IBRO. Published by Elsevier Science Ltd.