INTERACTION OF CANDIDA ALBICANS WITH HUMAN LEUKOCYTES AND SERUM
INTERACTION OF CANDIDA ALBICANS WITH HUMAN LEUKOCYTES AND SERUM
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DOI：
10.1128/jb.98.3.996-1004.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
CLINE, MJ
中科院分区：
文献类型：
--
作者：
LEHRER, RI;CLINE, MJ
本文报道了一种基于亚甲蓝对非活性白色念珠菌的鉴别染色的杀念珠菌活性的定量测定法，并应用于正常人和真菌及其它感染患者白细胞的研究。血清因子对C.白色念珠菌，但缺乏直接杀念珠菌活性。正常人中性粒细胞（38项研究）杀死29.0 ± 7.4%的摄入C。嗜酸性粒细胞和单核细胞杀死的百分比较小。杀中性粒细胞念珠菌活性不需要白细胞合成蛋白质或核糖核酸，但可被厌氧条件、氰化钾和秋水仙碱抑制。1例遗传性髓过氧化物酶缺乏症患者和3例慢性肉芽肿病患儿的白细胞吞噬C。白色念珠菌通常，但未能杀死他们。我们的数据表明，中性粒细胞可以发挥重要的作用，在抗念珠菌感染和溶酶体酶髓过氧化物酶及其氧化剂底物过氧化氢的中性粒细胞的杀念珠菌活性的主要参与者。
A quantitative assay of candidacidal activity based on differential staining of non-viableCandida albicansby methylene blue was developed and applied to studies of leukocytes from normal individuals and patients with fungal and other infections. Serum factors were necessary for optimal phagocytosis ofC. albicansbut lacked direct candidacidal activity. Normal human neutrophils (38 studies) killed 29.0 ± 7.4% of ingestedC. albicansin 1 hr. Eosinophils and monocytes killed a smaller percentage. Neutrophil candidacidal activity did not require protein or ribonucleic acid synthesis by the leukocyte but was inhibited by anaerobic conditions, potassium cyanide, and colchicine. Leukocytes of a patient with hereditary myeloperoxidase deficiency and of three children with chronic granulomatous disease phagocytizedC. albicansnormally, yet failed to kill them. Our data suggest that the neutrophil can play an important role in resistance toCandidainfection and that the lysosomal enzyme myeloperoxidase and its oxidant substrate hydrogen peroxide are the major participants in neutrophil candidacidal activity.