Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - A comparison of different tissue sources
Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - A comparison of different tissue sources
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DOI：
10.1016/j.jcms.2011.05.004
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发表时间：
2012-06-01
影响因子：
3.1
通讯作者：
Schlegel, Karl Andreas
中科院分区：
文献类型：
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作者：
Stockmann, Philipp;Park, Jung;Schlegel, Karl Andreas
由于供体侧的并发症和骨替代材料缺乏成骨特性，在组织工程的范围内，越来越需要一种替代传统骨移植的方法。本动物实验比较了脂肪来源(AD)、骨膜来源(PD)和骨髓来源(BM)间充质干细胞(MSC)的体内成骨能力。组织来源的自体间充质干/祖细胞经体外扩增后诱导分化为成骨细胞。将体外培养的细胞种植在胶原支架上，然后将其添加到21只家猪新鲜创造的单皮质颅骨缺损处。纯胶原支架作为对照。在特定时间点处死动物，用组织形态计量学方法定量分析骨形成情况。比较各组的骨体积/总缺损区体积(BV/TV)和新生骨矿化率。在创面愈合的早期，直到30d，试验性缺损区的骨再生情况并不比对照缺损区好，但在后期，试验性缺损区的骨愈合过程比对照缺损区加快。与对照组相比，所有试验组在90天后均表现出完全的骨性愈合。在观察期内，不同实验缺损区之间的BV/TV和新骨矿化程度无显著差异。不考虑MSC的组织来源，细胞移植到单皮质骨缺损后，骨愈合的速度和模式是相似的。结果表明，体外扩增后自体AD-MSC、PD-MSC和BM-MSC移植在引导骨缺损再生方面的效果无显著差异。(C)2011年欧洲颅颌面部外科协会。
Due to donor side morbidity and the absence of osteogenic properties in bone substitutes, there is a growing need for an alternative to traditional bone grafting within the scope of tissue engineering. This animal study was conducted to compare the in vivo osteogenic potential of adipose-derived (AD), periosteum-derived (PD) and bone marrow-derived (BM) mesenchymal stem/progenitor cells (MSC). Autologous mesenchymal stem/progenitor cells of named tissue origin were induced into osteogenic differentiation following in vitro cell expansion. Ex vivo cultivated cells were seeded on a collagen scaffold and subsequently added to freshly created monocortical calvarial bone defects in 21 domestic pigs. Pure collagen scaffold served as a control defect. The animals were sacrificed at specific time points and de novo bone formation was quantitatively analyzed by histomorphometry. Bone volume/total defect volume (BV/TV) and the mineralization rate of newly formed bone were compared among the groups. In the early stages of wound healing, up to 30 days, the test defects did not show better bone regeneration than those in the control defect, but the bone healing process in the test defects was accelerated in the later stage compared to those in the control defect. All the test defects showed complete osseous healing after 90 days compared to those in the control defect. During the observation period, no significant differences in BV/TV and mineralization of newly formed bone among the test defects were observed. Irrespective of the tissue sources of MSC, the speed and pattern of osseous healing after cell transplantations into monocortical bone defects were comparable. Our results indicate that the efficiency of autologous AD-MSC, PD-MSC and BM-MSC transplantation following ex vivo cell expansion is not significantly different for the guided regeneration of bone defects. (C) 2011 European Association for Cranio-Maxillo-Facial Surgery.