Serologic confirmation of Ehrlichia equi and Borrelia burgdorferi infections in horses from the northeastern United States
Serologic confirmation of Ehrlichia equi and Borrelia burgdorferi infections in horses from the northeastern United States
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DOI：
10.2460/javma.2000.217.1045
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发表时间：
2000-10-01
期刊：
影响因子：
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通讯作者：
Fikrig, E
中科院分区：
文献类型：
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作者：
Magnarelli, LA;Ijdo, JW;Fikrig, E
目的-确定生活在美国东北部有贝氏疏螺旋体病临床症状或粒细胞埃立克体病临床症状的马是否有可检测到的伯氏疏螺旋体和埃立克体病的血清抗体。设计-前瞻性研究。动物-来自51个临床正常马，14个有临床症状的马和17个有粒细胞埃立克体征的马的血清样本。过程-血清B或E抗体用ELISA法，免疫印迹分析，或间接荧光抗体(IFA)染色进行检测。结果：在所测试的82个血清样本中，37个(45.1%)和13个(15.9%)有抗伯氏疏螺螺旋体或E马立克抗体。分别进行了分析。试验结果表明，12匹马同时接触了这两种药物，其中11匹马患有粒细胞性埃立克体病。ELISA定期检测伯氏杆菌重组蛋白(P)抗原p29、p37、p39和p41-G的抗体。免疫印迹分析的使用证实了ELISA法的结果，表明了对分子质量主要为31、34、37、39、41、58和93 kD的全细胞伯氏杆菌抗原的抗体反应性。结论和临床相关性-生活在扁虱(肩部硬蜱)丰富地区的马有时会接触到多种病原体。即使在血清样本中检测到针对两种病原体的抗体，使用ELISA对特定重组疏螺旋体抗体的分析也可以帮助区分患有疏螺旋体病和粒细胞性埃立克体病的马。使用免疫印迹分析是敏感的，与ELISA或IFA程序一起，适合于确认每种疾病的临床诊断。
Objective-To determine whether horses living in tick-infested areas of northeastern United Stales with clinical signs of borreliosis or granulocytic ehrlichiosis had detectable serum antibodies to both Borrelia burgdorferi and Ehrlichia equi.Design-Prospective study.Animals-Serum samples from 51 clinically normal horses, 14 horses with clinical signs of borreliosis, and 17 horses with clinical signs of granulocytic ehrlichiosis.Procedure-Serum B burgdorferi or E equi antibodies were measured by use of an ELISA, immunoblot analysis, or indirect fluorescent antibody (IFA) staining.Results-Of the 82 serum samples tested, 37 (45.1%) and 13 (15.9%) had detectable antibodies to B burgdorferi or E equi, respectively. Test results indicated that 12 horses had been exposed to both agents, 11 of these horses had granulocytic ehrlichiosis. The ELISA regularly detected antibodies to the following recombinant protein (p) antigens of B burgdorferi: p29, p37, p39, and p41-G. The use of immunoblot analysis confirmed ELISA results by indicating antibody reactivities to antigens of whole-cell B burgdorferi having molecular masses of predominantly 31, 34, 37, 39, 41, 58, and 93 kd.Conclusions and Clinical Relevance-Horses living in areas where ticks (Ixodes scapularis) abound are sometimes exposed to multiple pathogens. Analyses for specific recombinant borrelial antibodies using an ELISA can help separate horses with borreliosis from those with granulocytic ehrlichiosis, even when antibodies to both etiologic agents are detected in serum samples. Analysis using immunoblots is sensitive, and along with ELISA or IFA procedures, is suitable for confirming a clinical diagnosis of each disease.