Ultraviolet difference spectral study of the interaction of DNA with platinum complexes
Ultraviolet difference spectral study of the interaction of DNA with platinum complexes
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DNA与铂配合物相互作用的紫外差光谱研究
DOI：
10.1016/s0020-1693(00)84165-1
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发表时间：
1980
影响因子：
2.8
通讯作者：
Y. Kidani
中科院分区：
文献类型：
--
作者：
K. Inagaki;Y. Kidani
紫外差光谱数据提供了有用的信息，以区分抗肿瘤活性和非活性铂配合物之间的结合模式。用铂络合物处理的DNA样品的紫外差光谱显示出由于核酸碱基的电子分布的变化而在295 nm处的谱带和由于DNA的二级结构的变化而在270 nm处的谱带。吸光度比ΔA270/ΔA295是铂与DNA结合引起的DNA二级结构变化的指标。抗肿瘤活性铂配合物诱导的DNA二级结构的变化大于抗肿瘤非活性铂配合物诱导的DNA二级结构的变化，并且抗肿瘤活性铂配合物的紫外差光谱行为相似。在这项研究中，对DNA中的鸟嘌呤残基的优先结合的趋势得到证实。用铂配合物处理的DNA样品的熔融曲线显示增色性降低和过渡宽度加宽。除trans-Pt（NH_3）_2Cl_2外，其它铂配合物均降低了熔融温度。
Ultraviolet difference spectral data provided useful information to differentiate the binding modes between antitumor-active and -inactive platinum complexes. The uv difference spectra for the DNA samples treated with platinum complexes exhibit a band at 295 nm due to the change in electron distribution of nucleic acid bases and a band at 270 nm due to the change in the secondary structure of DNA. The absorption ratio, ΔA270/ΔA295, is an index for the change of the secondary structure of DNA induced by binding with platinum. Changes in the DNA secondary structure induced by antitumor-active platinum complexes are greater than those induced by antitumor-inactive platinum complexes, and the uv difference spectral behavior of antitumor-active platinum complexes is similar. A tendency for preferential binding toward the guanine residues in DNA was confirmed in this study. Melting profiles for the DNA samples treated with platinum complexes showed a decrease in hyperchromicity and a broadening of transition width. The platinum complexes, other thantrans-Pt(NH3)2Cl2, decreased the melting temperature.