High resolution ex vivo magnetic resonance imaging of in situ coronary and aortic atherosclerotic plaque in a porcine model
High resolution ex vivo magnetic resonance imaging of in situ coronary and aortic atherosclerotic plaque in a porcine model
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DOI：
10.1016/s0021-9150(99)00386-x
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发表时间：
2000-06-01
期刊：
影响因子：
5.3
通讯作者：
Badimon, JJ
中科院分区：
文献类型：
--
作者：
Worthley, SG;Helft, G;Badimon, JJ
动脉粥样硬化斑块的组成是斑块破裂和急性血栓形成的发病机制的核心。因此，需要动脉粥样硬化病变的精确成像和表征。尽管没有理想的动脉粥样硬化动物模型，但猪模型被认为最接近于人类动脉粥样硬化。我们报告的可行性磁共振成像和表征动脉粥样硬化病变的原位冠状动脉和动脉瘤在体外设置和验证这与组织病理学。在尤卡坦小型猪（n = 4）中通过致动脉粥样硬化饮食（6个月）和球囊损伤的组合诱导冠状动脉和主动脉粥样硬化。所有冠状动脉在完整心脏上进行离体成像，保留其路线的曲率。主动脉也进行了MR成像。冠状动脉（n = 54）和冠状动脉（n = 43）的MR图像与匹配的组织病理学切片相关联。MR成像准确地表征了复杂的动脉粥样硬化病变，包括钙化、富脂、纤维细胞和出血区域。MR成像准确地确定了冠状动脉（r = 0.94，斜率：0.81）和腹主动脉（r = 0.94，斜率：0.81）的平均壁厚度以及主动脉斑块面积（r = 0.97，斜率：0.90）（P < 0.0001）。冠状动脉MR成像不受心脏中冠状动脉曲率的限制。在这个复杂动脉粥样硬化的实验猪模型中，MR成像准确地量化和表征冠状动脉和主动脉粥样硬化病变，包括血管壁。该模型可能有助于进一步研究动脉粥样硬化的MR成像。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
Atherosclerotic plaque composition is central to the pathogenesis of plaque disruption and acute thrombosis. Thus, there is a need for accurate imaging and characterization of atherosclerotic lesions. Even though there is no ideal animal model of atherosclerosis, the porcine model is considered to most closely resemble human atherosclerosis. We report the feasibility of MR imaging and characterizing of atherosclerotic lesions from in situ coronary arteries and aortas in an ex vivo setting and validate this with histopathology. Coronary and aortic atherosclerosis was induced in Yucatan mini-swine (n = 4) by a combination of atherogenic diet (6 months) and balloon injury. All coronary arteries were imaged ex vivo on the intact heart, preserving the curvature bf their course. The aorta also underwent MR imaging. The MR images were correlated with the matched histopathology sections for both the coronary arteries (n = 54) and the aortas (n = 43). MR imaging accurately characterized complex atherosclerotic lesions, including calcified, lipid rich, fibrocellular and hemorrhagic regions. Mean wall thickness for the coronary arteries (r = 0.94, slope: 0.81) and aortas (r = 0.94, slope: 0.81) as well as aortic plaque area (r = 0.97, slope: 0.90) was accurately determined by MR imaging (P < 0.0001). Coronary artery MR imaging is not limited by the curvature of the coronary arteries in the heart. MR imaging accurately quantifies and characterizes coronary and aortic atherosclerotic lesions, including the vessel wall, in this experimental porcine model of complex atherosclerosis. This model may be useful for future study of MR imaging of atherosclerosis in vivo. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.