FREE-RADICAL DAMAGE TO LIPIDS, AMINO-ACIDS, CARBOHYDRATES AND NUCLEIC-ACIDS DETERMINED BY THIOBARBITURIC ACID REACTIVITY

FREE-RADICAL DAMAGE TO LIPIDS, AMINO-ACIDS, CARBOHYDRATES AND NUCLEIC-ACIDS DETERMINED BY THIOBARBITURIC ACID REACTIVITY
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DOI:
10.1016/0020-711x(82)90050-7
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发表时间:
1982-01-01
期刊:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY
影响因子:
--
通讯作者:
GUTTERIDGE, JMC
GUTTERIDGE, JMC
中科院分区:
其他
文献类型:
--
作者:
GUTTERIDGE, JMC

文献摘要

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The thiobarbituric acid (TBA) reaction, widely applied to the detection of autoxidation in polyunsaturated fatty acids, can be used to measure free-radical damage to amino acids, carbohydrates and nucleic acids. In all of these systems, malondialdehyde (MDA) is predominently formed from intermediate precursor molecules which break down during the acid-heating stage of the TBA test. The acid reagent used to bring about these decompositions appears to be critical.