ELEVATED RELEASE OF TUMOR-NECROSIS-FACTOR-ALPHA AND INTERFERON-GAMMA BY BRONCHOALVEOLAR LEUKOCYTES FROM PATIENTS WITH BRONCHIAL-ASTHMA
ELEVATED RELEASE OF TUMOR-NECROSIS-FACTOR-ALPHA AND INTERFERON-GAMMA BY BRONCHOALVEOLAR LEUKOCYTES FROM PATIENTS WITH BRONCHIAL-ASTHMA
复制标题
DOI：
10.1164/ajrccm/147.2.291
复制
发表时间：
1993-02-01
期刊：
影响因子：
--
通讯作者：
TEODORCZYKINJEYAN, JA
中科院分区：
文献类型：
--
作者：
CEMBRZYNSKANOWAK, M;SZKLARZ, E;TEODORCZYKINJEYAN, JA
支气管肺泡灌洗（BAL）白细胞分泌的细胞因子被认为是肺部炎症和过敏反应的重要介质。本研究检测了来自支气管哮喘患者（n = 11）的BAL细胞培养物中肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）产生水平的定量变化。TNF α和IFN γ的分泌测定在完整的（未刺激的）和植物血凝素/佛波醇肉豆蔻酸乙酸酯（PHA+PMA）刺激的BAL白细胞培养物，并与对照培养物进行比较。在所有研究的患者中，背景和PHA + PMA诱导的TNF α和IFN γ分泌显著（p < 0.001）高于平行对照培养物。与BAL细胞制剂相反，患者外周血单核细胞（PBMC）分泌TNF α和IFN γ的能力与对照受试者没有差异。患者的BAL白细胞而非PBMC自发释放大量TNF α和IFN γ，提示在支气管哮喘的病理生理学中，这些细胞因子可能作为肺中的局部致病因子。
Bronchoalveolar lavage (BAL) leukocyte-secreted cytokines are considered to be important mediators of the inflammatory and allergic reactions in the lung This study examines quantitative changes in the level of tumor necrosis factor-alpha (TNFalpha) and interferon-gamma (IFNgamma) production in BAL cell cultures derived from patients (n = 11) with bronchial asthma. The secretion of TNFalpha and IFNgamma was determined in intact (unstimulated) and phytohemagglutinin/phorbol myristate acetate (PHA+PMA)-stimulated BAL leukocyte cultures and compared with that in control cultures. In all patients studied, the background and PHA + PMA-induced secretion of TNFalpha and IFNgamma was significantly (p < 0.001) higher than that in parallel control cultures. In contrast to BAL cell preparations, the capacity of TNFalpha and IFNgamma secretion by patients' peripheral blood mononuclear cells (PBMC) did not differ from that of control subjects. High spontaneous release of TNFalpha and IFNgamma by patients' BAL leukocytes, but not PBMC, suggest that in the pathophysiology of bronchial asthma, these cytokines may act as local pathogenic agents in the lung.