Characterization of an axonemal dynein heavy chain expressed early in airway epithelial ciliogenesis.
Characterization of an axonemal dynein heavy chain expressed early in airway epithelial ciliogenesis.
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气道上皮纤毛发生早期表达的轴丝动力蛋白重链的表征。
DOI：
10.1165/ajrcmb.23.6.4045
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Collier,AM
中科院分区：
文献类型：
--
作者：
Reed,W;Carson,JL;Moats-Staats,BM;Lucier,T;Hu,P;Brighton,L;Gambling,TM;Huang,CH;Leigh,MW;Collier,AM
气道上皮成熟和活力最明显的证据是运动纤毛的存在。为了产生气道成熟、损伤和修复的遗传和抗原标记，我们对气道上皮基因的表达进行了表征，该基因由两个人类表达的序列标签鉴定，该序列标签编码与无脊椎动物纤毛动力蛋白重链（DHC）序列相似的肽。分子分析表明，该基因有一个非常大的RNA转录本，编码一个非常高分子量的多肽，具有动力蛋白重链的生化特性。基因转录物的表达与组织中纤毛细胞的存在相关，基因产物的免疫组织化学定位证实其存在于成熟气道上皮的纤毛中。在体内发生纤毛发生的上皮中，基因转录本的表达先于纤毛发生的上皮，基因产物存在于细胞质和非纤毛细胞的顶端边缘。这些数据表明，该基因编码的轴突DHC在纤毛发生早期表达，在纤毛出现之前。
The most conspicuous evidence of airway epithelial maturation and vitality is the presence of motile cilia. In an effort to generate genetic and antigenic markers of airway maturation, injury, and repair, we characterized airway epithelial expression of a gene identified by two human expressed sequence tags that encoded peptides with sequence similarity to an invertebrate ciliary dynein heavy chain (DHC). Molecular analyses showed that the gene has a very large RNA transcript that encodes a very high molecular weight polypeptide with biochemical properties that are characteristic of a dynein heavy chain. Expression of the gene transcript correlated with the presence of ciliated cells in tissues, and immunohistochemical localization of the gene product confirmed its presence in the cilia of mature airway epithelium. In epithelium undergoing ciliogenesisex vivo, expression of the gene transcript preceded ciliation of the epithelium and the gene product was present in the cytoplasm and at the apical border of nonciliated cells. These data suggested that the gene encodes an axonemal DHC that is expressed early during ciliogenesis, before the appearance of cilia.