Differential effects of single and repeated administration of gonadotropins on testosterone production and steroidogenic enzymes in Leydig cell populations.
Differential effects of single and repeated administration of gonadotropins on testosterone production and steroidogenic enzymes in Leydig cell populations.
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单次和重复施用促性腺激素对 Leydig 细胞群中睾酮产生和类固醇生成酶的不同影响。
DOI：
10.1016/s0021-9258(18)33789-x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
A. Payne
中科院分区：
文献类型：
--
作者：
P. O’Shaughnessy;A. Payne
类固醇生成酶活性的材料和方法测量。a）e~-38-Hydryrtemld &？氢化酶-异构酶通过测量15- 3 α-羟基甾体类化合物的活性来测定15- 3 α-羟基甾体类化合物的活性。在37 ℃下，在含有1 μ Ci [1H]普烯醇酮的玻璃试管中进行15分钟的孵育，所述[1H]普烯醇酮具有不同浓度的非放射性活性的普烯醇酮。溶于0.03 ml二甲基亚砜（DMSO）和0.87 ml 50 M磷酸盐缓冲液（pH7.4）中，含有0.5 mM NAO。通过加入0.1 ml适当的均烯酸盐引发反应，并通过加入0.1 ml 1 N NaMI终止反应。[2016 - 05 - 25] Pregnenolone（50 up. 5000 dpa）和？[l* C]丙孕酮
MATERIALS AND METHODS'Measurement O f Steroidogenic Enzfle Activity. a) e~-38-Hydraryrtemld &? hydrogenase-Isomer-ase Actjvlt. The activity af 15-3a-hydmxyrteroid dehydrogensre-irrmerarL was determined by measuring tk conversion of ['H] pregnenolane to ['H] pmgerterone as described yeulourly (131. InCUbdtiOnl were performed for 15 nin at 37'C in glass tubel containing 1 uCi [Hlpreqnenelone wlth varylng concentrations O f non-radloactlve pregnenolone. dissolved in 0.03 nl dlsethylsulfoxide (DMSO1 and 0.87 ml 50 M phosphate buffer (pH 7.4) contalnlng 0.5 mM NAO'. The reaction was initiated by the addltlon of 0.1 ml of the appropriate homo enate and stopped by the addition Of 0.1 m1 1N NaMI.['kC] Pregnenolone(50 up. 5000dpa) and? l* C] pmgrrtorone