Antifungal susceptibility of Cryptococcus neoformans and Cryptococcus gattii isolates from decayed wood of trunk hollows of Ficus religiosa and Syzygium cumini trees in north-western India
Antifungal susceptibility of Cryptococcus neoformans and Cryptococcus gattii isolates from decayed wood of trunk hollows of Ficus religiosa and Syzygium cumini trees in north-western India
复制标题
DOI：
10.1093/jac/dkm192
复制
发表时间：
2007-08-01
影响因子：
5.2
通讯作者：
Chandy, Rachel
中科院分区：
文献类型：
--
作者：
Khan, Zia U.;Randhawa, Harbans S.;Chandy, Rachel
背景：我们提供了从榕树和蒲桃树树干空心的腐烂木材中培养的新型隐球菌（血清型 A，n = 117）和格特隐球菌（血清型 B，n = 65）的环境分离株的抗真菌敏感性数据。方法：对两性霉素 B、氟康唑、酮康唑、伊曲康唑和使用Etest测定伏立康唑。 48 小时后按照制造商提供的指南读取 MIC。结果：新型隐球菌分离株的 MIC(90) 和敏感性范围如下：两性霉素 B 0.094 (0.004-0.25) mg/L，氟康唑 4 (0.032-12) mg/L，0.094 (0.004-0.75)伊曲康唑为 0.064 (0.002-0.19) mg/L，伏立康唑为 0.047 (0.006-0.125) mg/L，而对于格特隐球菌分离株，两性霉素 B 为 0.125 (0.023-0.5) mg/L，8 (0.032-16) mg/L（氟康唑）、0.75（0.006-2）mg/L（伊曲康唑）、0.125（0.003-0.19）mg/L（酮康唑）和 0.094（0.004-0.125）mg/L（伏立康唑）。 MIC (mg/L) 几何平均值的比较表明，格特隐球菌比新型隐球菌对两性霉素 B（0.075 与 0.051 9 P = 0.0003）、氟康唑（2.9,12 与 2.316，P = 0.003）、伊曲康唑（0.198 与 0.0344，P < 0.0001）、酮康唑（0.072 vs 0.037，P < 0.0001）和伏立康唑（0.045 vs 0.023，P < 0.0001）。结论：本研究获得的抗真菌药敏数据表明，A 型新型隐球菌和格特隐球菌环境分离株存在原发耐药。 B 血清型很少见，B 血清型分离株比 A 血清型分离株更不易感。
Background: We present antifungal susceptibility data on environmental isolates of Cryptococcus neoformans (serotype A, n = 117) and Cryptococcus gattii (serotype B, n = 65) cultured from decayed wood of trunk hollows of Ficus religiosa and Syzygium cumini trees.Methods: Susceptibilities to amphotericin B, fluconazole, ketoconazole, itraconazole and voriconazole were determined by using Etest. The MICs were read after 48 h as per the guidelines provided by the manufacturer.Results: The MIC(90)s and susceptibility ranges for C. neoformans isolates were as follows: 0.094 (0.004-0.25) mg/L for amphotericin B, 4 (0.032-12) mg/L for fluconazole, 0.094 (0.004-0.75) mg/L for itraconazole, 0.064 (0.002-0.19) mg/L for ketoconazole, and 0.047 (0.006-0.125) mg/L for voriconazole, whereas for C. gattii isolates these were 0.125 (0.023-0.5) mg/L for amphotericin B, 8 (0.032-16) mg/L for fluconazole, 0.75 (0.006-2) mg/L for itraconazole, 0.125 (0.003-0.19) mg/L for ketoconazole, and 0.094 (0.004-0.125) mg/L for voriconazole. A comparison of the geometric means of MICs (mg/L) revealed that C. gattii was less susceptible than C. neoformans to amphotericin B (0.075 versus 0.051 9 P = 0.0003), fluconazole (2.9,12 versus 2.316, P = 0.003), itraconazole (0.198 versus 0.0344, P < 0.0001), ketoconazole (0.072 versus 0.037, P < 0.0001), and voriconazole (0.045 versus 0.023, P < 0.0001).Conclusions: The antifungal susceptibility data obtained in this study indicate that the occurrence of primary resistance among environmental isolates of C. neoformans serotype A and C. gattii serotype B is rare, and serotype B isolates are less susceptible than serotype A isolates.