Organization of extracellular proteins on the connective tissue cell surface: relevance to cell-matrix interactions in vitro and in vivo.
Organization of extracellular proteins on the connective tissue cell surface: relevance to cell-matrix interactions in vitro and in vivo.
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DOI：
10.1111/j.1749-6632.1978.tb16795.x
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发表时间：
1978-06-20
影响因子：
5.2
通讯作者：
Crouch, E
中科院分区：
文献类型：
--
作者：
Bornstein, P;Duksin, D;Crouch, E
已经开发了一种模型，提出了一种细胞表面相关的蛋白质网络，由纤维连接蛋白和胶原蛋白的一部分，连接到一个基质的结缔组织细胞。胶原蛋白和纤连蛋白相互作用以及这些蛋白质类似地分布在成纤维细胞表面的观察结果支持了该模型。我们认为，这种外部网络直接或间接地与内部细胞骨架和细胞外基质相互作用，从而介导几种细胞特性，包括粘附，形状和运动性。由于病毒转化或由于用衣霉素（一种蛋白糖基化抑制剂）处理正常细胞而导致细胞表面纤连蛋白的丢失，可能导致在这些情况下观察到的粘附力降低和形态改变。因此，我们预测，这些性质的变化与病毒转化的细胞，有丝分裂细胞，和细胞与蛋白水解酶处理的外部蛋白质网络的改变有关。
A model has been developed that proposes a cell surface-associated protein meshwork, composed in part of fibronectin and collagen, for a connective tissue cell attached to a substratum. In support of this model are the observations that collagen and fibronectin interact and that these proteins are similarly distributed on the fibroblast cell surface. We suggest that this external meshwork interacts directly or indirectly with the internal cytoskeleton and with the extracellular matrix and thereby mediates several cellular properties, including adhesion, shape, and motility. Loss of cell surface fibronectin as a result of viral transformation, or due to treatment of normal cells with tunicamycin, an inhibitor of protein glycosylation, may contribute to the reduced adhesion and altered morphology observed in these circumstances. We therefore predict that the changes in these properties observed with virally transformed cells, mitotic cells, and cells treated with proteolytic enzymes are related to alterations in the external protein meshwork.