Immunosensor using surface plasmon resonance for C-reactive protein detection
Immunosensor using surface plasmon resonance for C-reactive protein detection
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DOI：
10.5796/electrochemistry.74.153
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发表时间：
2006-02-01
期刊：
影响因子：
2.5
通讯作者：
Suzuki, H
中科院分区：
文献类型：
--
作者：
Casa, E;Kurosawa, C;Suzuki, H
将便携式表面等离子体共振（SPR）仪与微流控SPR传感器芯片（MSSC）的抗人C-反应蛋白单克隆抗体（AbCRP）固定金表面相结合，研制了一种检测人C-反应蛋白（CRP）的免疫传感器。采用自组装技术，用4，4 '-二硫代二丁酸对MSSC金表面进行了化学修饰。随后，通过化学吸附将AbCRP固定在MSSC的化学活化金表面上，并使用免疫反应进行CRP测定。该开发的SPR方法对CRP浓度的检测限为1.0 μ g/ml。
An immunosensor for human C-reactive protein (CRP) detection was developed by combining a portable surface plasmon resonance (SPR) machine with an anti-human C-reactive protein monoclonal antibody (AbCRP)-immobilized gold surface of the microfluidic SPR sensor chip (MSSC). The gold surface of MSSC was chemically modified using self-assembly monolayer technique with 4,4'-dithiodibutyric acid. Subsequently, AbCRP was immobilized on the chemically-activated gold surface of the MSSC by chemical adsorption and CRP determinations were performed using immunoreaction. The detection limit for CRP concentration by this developed SPR method was 1.0 mu g/ml.