The regulatory function of LexA is temperature-dependent in the deep-sea bacterium Shewanella piezotolerans WP3.
The regulatory function of LexA is temperature-dependent in the deep-sea bacterium Shewanella piezotolerans WP3.
复制标题
LexA 的调节功能在深海细菌 Shewanella piezotolerans WP3 中具有温度依赖性
DOI：
10.3389/fmicb.2015.00627
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Jian H;Xiong L;He Y;Xiao X
SOS反应针对DNA损伤，并且在细菌结构域中是保守的。该反应由DNA结合蛋白莱克萨控制，其已在模型微生物如大肠杆菌中表征。然而，我们对它在深海细菌中的作用的了解是有限的。本研究通过构建莱克萨缺失菌株（WP3Δ莱克萨），并与野生型菌株进行比较，研究了莱克萨对耐压希瓦氏菌WP3（WP3）表型和基因转录的影响。WP3Δ莱克萨没有观察到生长缺陷。通过比较全基因组微阵列分析，分别在20 ° C和4°C下总共有481个和108个基因差异表达。群集运动实验和二甲基亚砜还原实验表明，莱克萨的功能与温度有关。莱克萨基因的转录在冷驯化和冷休克后均上调，表明莱克萨在低温下的高表达可能是其温度依赖性功能的原因。深海微生物S. piezotolerans WP 3是迄今为止唯一一种其SOS调节剂被证明受环境温度显著影响的细菌物种。我们的数据支持这一假设，SOS是一个强大的战略，细菌对各种环境压力。
The SOS response addresses DNA lesions and is conserved in the bacterial domain. The response is governed by the DNA binding protein LexA, which has been characterized in model microorganisms such as Escherichia coli. However, our understanding of its roles in deep-sea bacteria is limited. Here, the influence of LexA on the phenotype and gene transcription of Shewanella piezotolerans WP3 (WP3) was investigated by constructing a lexA deletion strain (WP3ΔlexA), which was compared with the wild-type strain. No growth defect was observed for WP3ΔlexA. A total of 481 and 108 genes were differentially expressed at 20 and 4°C, respectively, as demonstrated by comparative whole genome microarray analysis. Furthermore, the swarming motility and dimethylsulfoxide reduction assay demonstrated that the function of LexA was related to temperature. The transcription of the lexA gene was up-regulated during cold acclimatization and after cold shock, indicating that the higher expression level of LexA at low temperatures may be responsible for its temperature-dependent functions. The deep-sea microorganism S. piezotolerans WP3 is the only bacterial species whose SOS regulator has been demonstrated to be significantly influenced by environmental temperatures to date. Our data support the hypothesis that SOS is a formidable strategy used by bacteria against various environmental stresses.