Up-regulation of Anxa2 gene promotes proliferation and invasion of breast cancer MCF-7 cells
Up-regulation of Anxa2 gene promotes proliferation and invasion of breast cancer MCF-7 cells
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Anxa2基因上调促进乳腺癌MCF-7细胞增殖和侵袭
DOI：
10.1111/j.1365-2184.2012.00820.x
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发表时间：
2012-06-01
影响因子：
8.5
通讯作者：
Niu, R.
中科院分区：
文献类型：
--
作者：
Wu, B.;Zhang, F.;Niu, R.
目的：与亲本野生型细胞相比，具有耐药特性的乳腺癌细胞的转移能力更高。膜联蛋白 A2 (Anxa2) 是一种 36 kDa 钙依赖性磷脂结合蛋白，其表达在转移性肿瘤中增加，并被发现与耐药性和转移表型相关。材料、方法和结果：在本研究中，我们发现Anxa2的上调与MCF-7乳腺癌细胞的体外和体内迁移和侵袭能力增强相关。 Western blot 分析显示，接触化疗药物可能会诱导 Anxa2 表达升高。此外，我们的数据表明Anxa2可能通过激活Erk1/2信号通路增加c-myc和cyclin D1的表达来影响MCF-7细胞的增殖、迁移和侵袭。结论：我们的研究结果表明，Anxa2 的上调可能通过调节许多相关下游靶基因在调节乳腺癌 MCF-7 细胞的增殖和侵袭中发挥重要作用。
Objective: The metastatic ability of breast cancer cells with chemoresistant properties is higher when compared to that of their parental wild- type cells. Expression of AnnexinA2 ( Anxa2), a 36- kDa calcium- dependent phospholipid binding protein, is increased in metastatic tumours and has been found to be associated with the phenotype of drug resistance and metastasis. Materials and Methods and Results: In the present study, we found that up- regulation of Anxa2 correlates with enhanced migration and invasion ability of MCF- 7 breast cancer cells both in vitro and in vivo. Western blot analysis revealed that exposure to chemotherapeutic drugs may induce elevated expression of Anxa2. In addition, our data have shown that Anxa2 might influence proliferation, migration and invasion of MCF- 7 cells by increasing expression of c- myc and cyclin D1 via activation of Erk1/ 2 signalling pathways. Conclusion: Our findings suggest that up- regulation of Anxa2 may play an important role in modulating proliferation and invasion of breast cancer MCF- 7 cells through regulation of many relevant downstream target genes.