A potential role for interleukin-8 in the metastatic phenotype of breast carcinoma cells
A potential role for interleukin-8 in the metastatic phenotype of breast carcinoma cells
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DOI：
10.1016/s0002-9440(10)64005-9
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发表时间：
2001-02-01
影响因子：
6
通讯作者：
Furcht, LT
中科院分区：
文献类型：
--
作者：
De Larco, JE;Wuertz, BRK;Furcht, LT
这项研究表明，乳腺癌细胞系的转移潜力和它们的异位表达白细胞介素-8（IL-8）之间有很强的相关性。相关性存在于IL-8释放的组成性和诱导性水平。还观察到细胞形态、转移潜能和IL-8谱之间的相关性。转移细胞系呈梭形，而非转移细胞系呈上皮样。采用原位自发转移模型检测了两个乳腺癌细胞系的转移潜能。转移性细胞形成快速生长的低分化原发性肿瘤，其转移，非转移性细胞形成快速生长的分化原发性肿瘤，其不产生可检测的转移。亲本细胞和从原发性和转移性肿瘤发育的细胞培养物的IL-8表达的比较表明，由来自原发性肿瘤的培养细胞释放的IL-8高于亲本细胞的IL-8，并且由来自转移性肺肿瘤的培养细胞释放的IL-8大于由来自原发性肿瘤的培养细胞释放的IL-8。这些数据表明细胞的转移表型与其IL-8表达之间的强相关性，表明IL-8在促进乳腺肿瘤细胞的转移潜力中的作用。
This study shows a strong correlation between the metastatic potentials of breast carcinoma cell lines and their ectopic expression of interleukin-8 (IL-8). Correlations exist for both constitutive and induced levels of IL-8 released, A correlation was also observed between cell morphology, metastatic potential, and IL-8 profile. Metastatic lines are fusiform in appearance, whereas, nonmetastatic lines are epithelioid, The metastatic potential of two breast carcinoma lines was examined using an orthotopic model of spontaneous metastasis. Metastatic cells formed rapidly growing, poorly differentiated primary tumors that metastasized, Nonmetastatic cells formed rapidly growing differentiated primary tumors that did not produce detectable metastases. Comparison of IL-8 expression by the parental cells and cell cultures developed from primary and metastatic tumors, demonstrates that IL-8 released by cultured cells from the primary tumor is higher than that of the parental cells, and IL-8 released by cultured cells derived from the metastatic lung tumors is greater than that released by cultured cells derived from the primary tumor. These data demonstrate a strong correlation between the metastatic phenotype of a cell and its IL-8 expression, suggesting a role for IL-8 in promoting the metastatic potential of breast tumor cells.