Does reduced usage of antibiotics in livestock production mitigate the spread of antibiotic resistance in soil, earthworm guts, and the phyllosphere?
Does reduced usage of antibiotics in livestock production mitigate the spread of antibiotic resistance in soil, earthworm guts, and the phyllosphere?
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DOI：
10.1016/j.envint.2019.105359
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发表时间：
2020-02
影响因子：
11.8
通讯作者：
Shuyidan Zhou;Dong Zhu;M. Giles;T. Daniell;R. Neilson;Xiao-Ru Yang
中科院分区：
文献类型：
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作者：
Shuyidan Zhou;Dong Zhu;M. Giles;T. Daniell;R. Neilson;Xiao-Ru Yang
抗生素在畜牧业中的广泛使用被认为是动物抗生素耐药基因(ARGs)选择的重要原因。因此，全球都在推动减少农业部门抗生素的使用。然而，减少在畜牧业生产中使用抗生素是否会有效地减少ARGs的传播还没有确定。使用微观世界方法来确定添加抗生素水平降低的粪便或添加典型抗生素水平的粪便如何影响抗生素抗性基因在土壤、蚯蚓和叶圈之间的传播。与对照土壤相比，常规有机肥处理的蚯蚓和叶际土壤中精氨酸含量的增加幅度更大(P<； )。与对照相比，减少的抗生素粪便也增加了叶圈和土壤中ARG的丰度，但没有增加蚯蚓肠道中的ARG(P<； 0.05)。在土壤和蚯蚓肠道中，减量抗生素肥料对Args的富集量均低于常规肥料。这项研究已经确定了通过土壤-蚯蚓-叶圈系统的细菌转移是在用牲畜粪便施肥后在生境之间传播ARGs的一种潜在手段。
The overuse of antibiotics in animal husbandry is widespread and believed to significantly contribute to the selection of antibiotic resistance genes (ARGs) in animals. Thus, there is a global drive to reduce antibiotic use in the agricultural sector. However, it has not been established whether a reduction in the use of antibiotics in livestock production would be effective in reducing the spread of ARGs. A microcosm approach was used to determine how the addition of manure with either reduced antibiotic levels or with typical antibiotic levels could affect the spread of antibiotic resistance genes between soil, earthworms and the phyllosphere. When compared to the control soil, earthworm and phyllosphere samples had the greater increase in ARG abundance in conventional manure treatments (P< 0.05). Reduced antibiotic manure also enriched the abundance of ARGs in the phyllosphere and soil but not earthworm guts when compared to the control (P< 0.05). In both soil and earthworm guts, the enrichment of ARGs was lower in reduced antibiotic manure than in conventional manure. This study has identified bacterial transfer through the soil-earthworm-phyllosphere system as a potential means to spread ARGs between habitats after fertilization with livestock derived manures.