Analyses of associations between three positionally cloned asthma candidate genes and asthma or asthma-related phenotypes in a Chinese population.
Analyses of associations between three positionally cloned asthma candidate genes and asthma or asthma-related phenotypes in a Chinese population.
复制标题
中国人群中三个定位克隆哮喘候选基因与哮喘或哮喘相关表型的关联分析
DOI：
10.1186/1471-2350-10-123
复制
发表时间：
2009-12-01
影响因子：
--
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Zhou H;Hong X;Jiang S;Dong H;Xu X;Xu X
背景根据报道的连锁研究，位于不同染色体区域的六个哮喘候选基因 ADAM33、NPSR1、PHF11、DPP10、HLA-G 和 CYFIP2 已被定位克隆。对于 ADAM33、NPSR1 和 CYFIP2，已在中国和日本等东亚人群中研究了与哮喘或哮喘相关表型的关联。然而，对于 PHF11、DPP10 和 HLA-G，尚未在亚洲人群中进行关联研究。因此，本研究的目的是测试这三个定位克隆基因与中国人群哮喘或哮喘相关表型之间的关联。方法在 1183 个独立样本中，分别对 PHF11、DPP10 和 HLA-G 顶部区域的 2 个、5 个和 2 个单核苷酸多态性 (SNP) 进行基因分型。研究样本的选择基于哮喘受影响状态和以下三种哮喘相关表型中至少一种的极值：血清总免疫球蛋白E水平、支气管反应性试验和皮肤点刺试验。进行了单一SNP和单倍型分析。结果经过多重检测调整后，我们发现DPP10与支气管高反应性（BHR）和BHR哮喘显着相关；而 PHF11 与抗原阳性皮肤反应的关联以及 HLA-G 与 BHR 哮喘的关联仅具有名义上的显着性。 结论 我们的研究是第一个提供额外证据支持 DPP10 在影响中国人群哮喘或 BHR 中的作用的研究。
BackgroundSix asthma candidate genes, ADAM33, NPSR1, PHF11, DPP10, HLA-G, and CYFIP2, located at different chromosome regions have been positionally cloned following the reported linkage studies. For ADAM33, NPSR1, and CYFIP2, the associations with asthma or asthma-related phenotypes have been studied in East Asian populations such as Chinese and Japanese. However, for PHF11, DPP10, and HLA-G, none of the association studies have been conducted in Asian populations. Therefore, the aim of the present study is to test the associations between these three positionally cloned genes and asthma or asthma-related phenotypes in a Chinese population.MethodsTwo, five, and two single nucleotide polymorphisms (SNPs) in the identified top regions of PHF11, DPP10, and HLA-G, respectively, were genotyped in 1183 independent samples. The study samples were selected based on asthma affectation status and extreme values in at least one of the following three asthma-related phenotypes: total serum immunoglobulin E levels, bronchial responsiveness test, and skin prick test. Both single SNP and haplotype analyses were performed.ResultsWe found that DPP10 was significantly associated with bronchial hyperresponsiveness (BHR) and BHR asthma after the adjustment for multiple testing; while the associations of PHF11 with positive skin reactions to antigens and the associations of HLA-G with BHR asthma were only nominally significant.ConclusionOur study is the first one to provide additional evidence that supports the roles of DPP10 in influencing asthma or BHR in a Chinese population.