Promotion of the self-renewal capacity of human leukemia cells by sonic hedgehog protein.
Promotion of the self-renewal capacity of human leukemia cells by sonic hedgehog protein.
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发表时间：
2011-03
影响因子：
2
通讯作者：
N. Kawaguchi-Ihara;Yuki Okuhashi;M. Itoh;I. Murohashi;N. Nara;S. Tohda
中科院分区：
文献类型：
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作者：
N. Kawaguchi-Ihara;Yuki Okuhashi;M. Itoh;I. Murohashi;N. Nara;S. Tohda
背景Hedgehog(HH)信号参与癌细胞生长。然而，HH刺激对白血病细胞的影响尚不清楚。材料与方法检测重组Sonic Hedgehog(Shh)蛋白对1例B淋巴瘤和4株髓系白血病细胞株体外生长的影响。结果Shh刺激对5种细胞株的短期生长均无明显影响。然而，Shh在悬浮培养后促进克隆形成细胞的恢复，这表明在三种细胞系中都促进了白血病干细胞或祖细胞的扩增。一种细胞缺乏HH受体，另一种细胞内源性Shh表达，可能是Shh在这些病例中缺乏作用的原因。结论Shh刺激可促进白血病干细胞在某些细胞系中的自我更新能力。抑制HH信号转导可能代表白血病治疗的新途径。
BACKGROUND Hedgehog (Hh) signaling is involved in cancer cell growth. However, the effects of Hh stimulation on leukemia cells are unknown. MATERIALS AND METHODS The effects of recombinant sonic Hedgehog (Shh) protein on the in vitro growth of one B-lymphoma and four myeloid leukemia cell lines were examined. RESULTS Shh stimulation had no significant effect on the short-term growth of whole cell populations in any of the five cell lines. However, Shh promoted clonogenic cell recovery after suspension culture, suggesting promotion of leukemia stem or progenitor cell amplification in three cell lines. The lack of Hh receptors in one cell line and endogenous Shh expression in another were possible reasons for the lack of effects of Shh in these cases. CONCLUSION These results suggest that Shh stimulation promotes the self-renewal capacity of leukemia stem cells in some cell lines. Inhibition of Hh signaling could represent a novel therapeutic approach in leukemia.