Use of human adenovirus-based vectors for antigen expression in animals.
Use of human adenovirus-based vectors for antigen expression in animals.
复制标题
使用基于人腺病毒的载体在动物中表达抗原。
DOI：
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发表时间：
1989
影响因子：
3.8
通讯作者：
F. L. Graham
中科院分区：
文献类型：
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作者：
L. Prevec;Maiko Abel Schneider;Kenneth L. Rosenthal;L. Belbeck;J. Derbyshire;F. L. Graham
利用携带水泡性口炎病毒（VSV）糖蛋白基因并在培养的HeLa细胞中表达该基因的感染性重组人腺病毒5型（Ad5）载体AdG 12，来检查人Ad载体的插入表达的宿主范围。VSV糖蛋白表达在牛，犬和小鼠细胞感染AdG12时，在文化。这些细胞系对于人Ad5复制分别是允许的、非允许的和半允许的。通过皮下或口服途径向小牛、仔猪或狗施用AdG12载体导致产生高滴度的VSV中和抗体。小鼠腹腔注射载体产生中和抗体，并保护其免受随后的静脉内攻击与正常致死剂量的VSV。这项工作证明了人腺病毒载体在许多非人细胞系中用于抗原表达和在许多物种中用于诱导对递送的抗原的免疫应答的效用。
An infectious recombinant human adenovirus type 5 (Ad5) vector, AdG12, which carries the glycoprotein gene of vesicular stomatitis virus (VSV) and expresses that gene in cultured HeLa cells was used to examine the host range of insert expression by human Ad vectors. The VSV glycoprotein was expressed in bovine, canine and murine cells when infected with AdG12 in culture. These cell lines are respectively permissive, non-permissive and semi-permissive for human Ad5 replication. Administration of the AdG12 vector to calves, piglets or dogs by either the subcutaneous or oral route resulted in the production of high titres of neutralizing antibodies to VSV. Mice injected intraperitoneally with the vector produced neutralizing antibodies and were protected against subsequent intravenous challenge with normally lethal doses of VSV. This work demonstrates the utility of human adenoviral vectors for antigen expression in a number of non-human cell lines and for the induction of an immune response to the delivered antigen in a number of species.