Multiple gene genealogies and phenotypic characters differentiate several novel species of Mycosphaerella and related anamorphs on banana.
Multiple gene genealogies and phenotypic characters differentiate several novel species of Mycosphaerella and related anamorphs on banana.
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DOI：
10.3767/003158508x302212
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发表时间：
2008-06
期刊：
影响因子：
9.1
通讯作者：
Crous PW
中科院分区：
文献类型：
--
作者：
Arzanlou M;Groenewald JZ;Fullerton RA;Abeln EC;Carlier J;Zapater MF;Buddenhagen IW;Viljoen A;Crous PW
球腔菌属（Mycosphaerella）3种，即M. eumusae、M. fijiensis和M. musicola参与香蕉的Sigatoka病复合体。除了这三种主要病原体外，还描述了来自芭蕉的几种其他球腔菌属或其无性型。然而，对这些分类群知之甚少，并且对于这些物种中的大多数，没有文化或DNA可用于研究。在本研究中，我们收集了一个全球性的香蕉球腔菌菌株，并通过形态学和多基因核苷酸序列数据集进行比较。根据ITS区序列推断的系统发育关系，结合肌动蛋白基因、线粒体核糖体小亚基DNA和组蛋白H3基因的部分序列，揭示了香蕉上球腔菌属物种丰富的多样性。整合形态和分子数据集确认了20多种球腔菌属（包括香蕉（香蕉）上发生的。本研究再次证实了先前描述的存在尾孢apii，M。citri和M. thailandica，并鉴定了Mycosphaerella communis，M. lateralis和Passalora loranthi在这一寄主上。此外，还描述了8个新种，即香蕉盘孢菌Dissoconium musae、莫桑比克球腔菌Mycosphaerella mozambica、阿萨姆假尾孢Pseudocercospora assamensis、印度尼西亚假尾孢P.indonesiana、长孢假尾孢P.longispora、香蕉丝孢Stenella musae、香蕉丝孢S. musicola和S.昆士兰。
Three species of Mycosphaerella, namely M. eumusae, M. fijiensis, and M. musicola are involved in the Sigatoka disease complex of bananas. Besides these three primary pathogens, several additional species of Mycosphaerella or their anamorphs have been described from Musa. However, very little is known about these taxa, and for the majority of these species no culture or DNA is available for study. In the present study, we collected a global set of Mycosphaerella strains from banana, and compared them by means of morphology and a multi-gene nucleotide sequence data set. The phylogeny inferred from the ITS region and the combined data set containing partial gene sequences of the actin gene, the small subunit mitochondrial ribosomal DNA and the histone H3 gene revealed a rich diversity of Mycosphaerella species on Musa. Integration of morphological and molecular data sets confirmed more than 20 species of Mycosphaerella (incl. anamorphs) to occur on banana. This study reconfirmed the previously described presence of Cercospora apii, M. citri and M. thailandica, and also identified Mycosphaerella communis, M. lateralis and Passalora loranthi on this host. Moreover, eight new species identified from Musa are described, namely Dissoconium musae, Mycosphaerella mozambica, Pseudocercospora assamensis, P. indonesiana, P. longispora, Stenella musae, S. musicola, and S. queenslandica.