Reduced Vitellogenesis and Female Fertility in Gper Knockout Zebrafish.
Reduced Vitellogenesis and Female Fertility in Gper Knockout Zebrafish.
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DOI：
10.3389/fendo.2021.637691
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发表时间：
2021
影响因子：
5.2
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Wu XJ;Williams MJ;Kew KA;Converse A;Thomas P;Zhu Y
G蛋白偶联雌激素受体(GPER)在脊椎动物生殖中的作用仍存在争议。为了研究GPER的生殖作用，我们使用TALEN建立了一个GPER斑马鱼突变系(GPER−/−)。GPER突变雌鼠的生育力下降，胚胎产量下降40.85%，这与V期(730-750GPER m)排卵卵母细胞的数量显着减少有关。相应地，GPER−/−的卵巢中早期卵黄发生卵泡(400-450µm)的数量多于野生型(Wt)，这表明GPER−/−鱼随后的卵泡发育受到了阻碍。此外，GPER−/−雌性卵母细胞的血浆卵黄蛋白原水平降低，第三期卵黄发生卵母细胞的表皮生长因子受体(EGFR)表达低于wt卵母细胞。然而，肝细胞核雌激素受体水平没有改变，而卵巢卵泡中的雌激素水平升高。这些结果表明，GPER可能通过调节斑马鱼卵黄发生和EGFR的表达而参与调控卵泡发育。
The role G-protein coupled estrogen receptor (GPER) plays in vertebrate reproduction remains controversial. To investigate GPER’s reproductive role, we generated a gper zebrafish mutant line (gper−/−) using TALENs. Gper mutant females exhibited reduced fertility with a 40.85% decrease in embryo production which was associated with a significant decrease in the number of Stage V (730–750 μm) ovulated oocytes. Correspondingly, the number of early vitellogenic follicles (Stage III, 400–450 µm) in gper−/− ovaries was greater than that in wildtypes (wt), suggesting that subsequent follicle development was retarded in the gper−/− fish. Moreover, plasma vitellogenin levels were decreased in gper−/− females, and epidermal growth factor receptor (Egfr) expression was lower in Stage III vitellogenic oocytes than in wt counterparts. However, hepatic nuclear estrogen receptor levels were not altered, and estrogen levels were elevated in ovarian follicles. These results suggest that Gper is involved in the control of ovarian follicle development via regulation of vitellogenesis and Egfr expression in zebrafish.
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DOI：
10.1016/s0960-0760(01)00190-x
发表时间：
2002-02-01
影响因子：
4.1
作者：
Filardo, EJ
通讯作者：
Filardo, EJ
影响因子：
--
作者：
Filardo, EJ;Quinn, JA;Bland, KI
通讯作者：
Bland, KI
影响因子：
4.8
作者：
Maiti, K.;Paul, J. W.;Smith, R.
通讯作者：
Smith, R.
影响因子：
4
作者：
Aizen, Joseph;Thomas, Peter
通讯作者：
Thomas, Peter
影响因子：
4.8
作者：
Griffin, Lucinda B.;January, Kathleen E.;Callard, Gloria V.
通讯作者：
Callard, Gloria V.