Establishment and Characteristics of Porcine Preadipocyte Cell Lines Derived From Mature Adipocytes
Establishment and Characteristics of Porcine Preadipocyte Cell Lines Derived From Mature Adipocytes
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DOI：
10.1002/jcb.22431
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发表时间：
2010-02-15
影响因子：
4
通讯作者：
Kano, Koichiro
中科院分区：
文献类型：
--
作者：
Nobusue, Hiroyuki;Kano, Koichiro
建立前脂肪细胞系，如3 T3-L1和3 T3-F442 A的发展，大大促进了在特定条件下脂肪细胞分化的分子机制的研究。这些细胞系大多来自小鼠胚胎，其他物种的前脂肪细胞系尚未在培养中保持足够长的时间以研究在各种条件下的分化。本研究首次报道了采用天花板培养法建立猪前脂肪细胞系。该细胞系可以广泛增殖，直到细胞融合并完全分化为成熟脂肪细胞，这取决于脂肪形成诱导。没有观察到它们的分化模式的变化（诱导它们的繁殖），并且它们已经成功地进行并分化了至少37代。该细胞系保持正常表型而不自发转化，即使在培养中长期维持后也是如此。这一结果为猪前脂肪细胞系的建立和替代人前脂肪细胞研究脂肪细胞分化和代谢机制提供了新的模型体系。J.细胞。109：542-552，2010. (C)2009威利-利斯公司
Development of established preadipocyte cell lines, such as 3T3-L1 and 3T3-F442A, greatly facilitated the study of molecular mechanisms of adipocyte differentiation under defined conditions. Most of these cell lines are derived from mouse embryos, and preadipocyte cell lines of other species have not yet been maintained in culture long enough to study differentiation under a variety of conditions. This is the first report on the establishment of porcine preadipocyte cell lines derived front mature adipocytes by a simple method, known as ceiling culture, for culturing mature adipocytes in vitro. This cell line can proliferate extensively until the cells become confluent and fully differentiated into mature adipocytes, depending on adipogenic induction. No changes in their differentiation pattern are observed (luring their propagation, and they have been successfully carried and differentiated for at least 37 passages. This cell line maintains a normal phenotype without transforming spontaneously, even after long-term maintenance in culture. This achievement may lead to easy establishment of porcine preadipocyte cell lines and novel model systems For studying the mechanisms of adipocyte differentiation and metabolism as a substitute for human preadipocytes. J. Cell. Biochem. 109: 542-552, 2010. (C) 2009 Wiley-Liss, Inc.