The metabolic status of diapause embryos of Artemia franciscana (SFB)
The metabolic status of diapause embryos of Artemia franciscana (SFB)
复制标题
DOI：
10.1086/physzool.69.1.30164200
复制
发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Sorgeloos, P
中科院分区：
文献类型：
--
作者：
Clegg, JS;Drinkwater, LE;Sorgeloos, P
卤虫广泛用于水产养殖和从分子生物学到进化和生态学等领域的基础研究。其生活史的一个关键特征涉及包囊胚胎的产生，其发育在原肠胚阶段停止（进入滞育）。这些带壳的胚胎被释放到水环境中，滞育持续到产生“激活胚胎”的合适条件终止，然后在条件允许时恢复发育。很少有人知道的代谢滞育胚胎，相反，激活胚胎，已被广泛研究。我们已经研究了选择功能的代谢滞育胚胎在实验室培养和收集从外地。虽然处于发育停滞状态，但新释放的滞育胚胎显示出进行旺盛的代谢。然而，随着滞育的继续，新陈代谢减慢，直到它的检测成为一个实验问题;新陈代谢有可能达到可逆的停顿。我们还介绍了滞育终止和恢复代谢的研究结果。最后，我们将表明，一个主要的蛋白质（p26），以前牵连作为一个潜在的分子伴侣在活化胚胎经历缺氧和热休克，表现出类似的滞育胚胎虽然缺乏直接的证据，该结果表明，p26的作用，可能是共同的机制与滞育的控制，以及在这个系统中的应激反应。
The brine shrimp Artemia franciscana is widely used in aquaculture and basic research in areas ranging from molecular biology to evolution and ecology. A key feature of its life history involves the production of encysted embryos whose development is halted (enters diapause) at the gastrula stage. These shelled embryos are released into the aqueous environment where diapause continues until terminated by suitable conditions that produce an ''activated embryo,'' which then can resume development when conditions permit. Very little is known about the metabolism of diapause embryos, in contrast to activated embryos, which have been studied extensively. We have examined selected features of metabolism in diapause embryos produced in laboratory cultures and collected from the field. Although in a state of developmental arrest, newly released diapause embryos are shown to carry on a vigorous metabolism. However, as diapause continues metabolism slows until its detection becomes an experimental problem; it is possible that metabolism comes to a reversible standstill. We also present results from studies on diapause termination and the resumption of metabolism. Finally, we will show that a major protein (p26), previously implicated as a potential molecular chaperone in activated embryos undergoing anoxia and thermal shock, behaves similarly in diapause embryos Although direct evidence is lacking, that result suggests a role for p26 that may be common to the mechanisms involved with the control of diapause as well as the stress response in this system.