Positional cloning and characterization of mouse mei8, a disrupted allele of the meiotic cohesin Rec8
Positional cloning and characterization of mouse mei8, a disrupted allele of the meiotic cohesin Rec8
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DOI：
10.1002/gene.20085
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发表时间：
2004-11-01
期刊：
影响因子：
1.5
通讯作者：
Schimenti, JC
中科院分区：
文献类型：
--
作者：
Bannister, LA;Reinholdt, LG;Schimenti, JC
在对ES细胞化学诱变引起的不育突变进行正向遗传筛选时，分离到一个新的突变mei 8。纯合子突变小鼠是不育的。突变雌性在生殖成熟时表现为卵巢发育不全和缺乏卵泡。受影响的男性睾丸较小，这是由于在减数分裂前期I精子发生受阻所致。mei 8的遗传作图和定位克隆导致Rec 8中突变的鉴定，Rec 8是酵母减数分裂特异性粘附素基因REC 8的同源物。Rec 8（mei 8）/Rec 8（mei 8）精母细胞减数分裂的分析表明，虽然重组和突触的启动发生，REC 8是完成和/或维持突触，姐妹染色单体的凝聚力，以及交叉的形成所必需的，因为它是在其他生物体。然而，与酵母和秀丽隐杆线虫不同，REC 8在减数分裂染色体上的定位对于轴向元件的组装是不需要的。创世纪40：184-194，2004。(C)2004 Wiley-Liss，Inc.
A novel mutation, mei8, was isolated in a forward genetic screen for infertility mutations induced by chemical mutagenesis of ES cells. Homozygous mutant mice are sterile. Mutant females exhibit ovarian dysgenesis and lack ovarian follicles at reproductive maturity. Affected males have small testes due to arrest of spermatogenesis during meiotic prophase I. Genetic mapping and positional cloning of mei8 led to the identification of a mutation in Rec8, a homolog of the yeast meiosis-specific cohesin gene REC8. Analysis of meiosis in Rec8(mei8)/Rec8(mei8) spermatocytes showed that, while initiation of recombination and synapsis occurs, REC8 is required for the completion and/or maintenance of synapsis, cohesion of sister chromatids, and the formation of chiasmata, as it is in other organisms. However, unlike yeast and Caenorhabditis elegans, localization of REC8 on meiotic chromosomes is not required for the assembly of axial elements. genesis 40:184-194, 2004. (C) 2004 Wiley-Liss, Inc.