THE NEURONAL ENDOPLASMIC-RETICULUM - ITS CYTO-CHEMISTRY AND CONTRIBUTION TO THE ENDOMEMBRANE SYSTEM .1. CELL-BODIES AND DENDRITES
THE NEURONAL ENDOPLASMIC-RETICULUM - ITS CYTO-CHEMISTRY AND CONTRIBUTION TO THE ENDOMEMBRANE SYSTEM .1. CELL-BODIES AND DENDRITES
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DOI：
10.1177/31.9.6309951
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
CATALDO, AM
中科院分区：
文献类型：
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作者：
BROADWELL, RD;CATALDO, AM
内质网（ER）及其对内膜系统的贡献（即，应用EM酶细胞化学研究了小鼠脑中神经元中的细胞器膜），以证明葡萄糖-6-磷酸酶（G6 β）活性。G6 β的磷酸水解活性是许多细胞类型中ER的众所周知的细胞化学标志物。在所用的不同底物中，G=6=P和甘露糖-6-磷酸是仅有的2种在神经元ER和与ER形态学相关的细胞器中观察到G6 β反应产物的底物。细胞体和树突中的G6 α活性始终定位于核膜、粗糙和光滑ER、板层体、膜下和亚表面池的管腔内，并且经常定位于高尔基体的顺囊内。G6 β反应性ER呈囊泡和小管网络状分布于胞体及其树突。可能的膜连续性之间的ER和其他反应性结构，包括高尔基体的顺式半。无论是G6 β活性还是反应性ER都与反式高尔基体囊泡或GERL [高尔基体-内质网-溶酶体]无关。因此，G6 β活性可作为核周和树突状ER及相关结构的可靠标志物。这些观察结果支持了ER是神经元内膜系统的组成部分的理论;它与细胞内隔室之间的膜或膜分子的转移，可输出蛋白质的包装和运输以及能量代谢有关。G6 β在轴突和终末ER中的活性在本研究的第2部分中被详细考虑。
The endoplasmic reticulum (ER) and its contribution to the endomembrane system (i.e., membranes of cell organelles) in the neuron have been investigated in brains of mice by applying EM enzyme cytochemistry for demonstration of glucose-6-phosphatase (G6Pase) activity. The phosphohydrolytic activity of G6Pase is a well-known cytochemical marker for the ER in numerous cell types. Of the different substrates employed, G=6=P and mannose-6-phosphate were the only 2 with which G6Pase reaction product was seen in the neuronal ER and organelles related morphologically to the ER. G6Pase activity in cell bodies and dendrites was localized consistently within the lumen of the nuclear envelope, rough and smooth ER, lamellar bodies, hypolemmal and subsurface cisternae and, frequently, in the cis saccules of the Golgi apparatus. The G6Pase reactive ER appeared as a network of saccules and tubules pervading the cell body and its dendrites. Possible membrane continuities were identified between the ER and the other reactive structures, including the cis half of the Golgi apparatus. Neither G6Pase activity nor reactive ER was associated with the trans Golgi saccules or GERL [Golgi-endoplasmic reticulum-lysosome]. G6Pase activity thus serves as a reliable marker for the perikaryal and dendritic ER and related structures. These observations support the theory that the ER is an integral component of the neuronal endomembrane system; it is associated with the transfer of membrane or membrane molecules among intracellular compartments, the packaging and transport of exportable protein, and energy metabolism. G6Pase activity in the ER of axons and terminals is considered in detail in part 2 of this study.