Assessment of SYBR Green I Dye-Based Fluorescence Assay for Screening Antimalarial Activity of Cationic Peptides and DNA Intercalating Agents
Assessment of SYBR Green I Dye-Based Fluorescence Assay for Screening Antimalarial Activity of Cationic Peptides and DNA Intercalating Agents
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DOI：
10.1128/aac.03266-14
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发表时间：
2015-05-01
影响因子：
4.9
通讯作者：
Varshneya, Grish C.
中科院分区：
文献类型：
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作者：
Bhatia, Rakesh;Gautam, Ankur;Varshneya, Grish C.
用于筛选抗疟化合物的 SYBR green I (SG) 染料荧光测定法基于寄生虫 DNA 的直接定量。我们发现，与 DNA 相互作用的阳离子细胞穿透肽 (CPP) 和嵌入剂与 SG 染料竞争结合 DNA。因此，与[H-3]次黄嘌呤掺入测定的读数不同，该测定对于上述DNA结合剂的抗疟活性的读数可能是错误的。对于 CPP，可以通过去除多余的肽来改善错误读数。
The SYBR green I (SG) dye-based fluorescence assay for screening antimalarial compounds is based on direct quantitation of parasite DNA. We show that DNA-interacting cationic cell-penetrating peptides (CPPs) and intercalating agents compete with SG dye to bind to DNA. Therefore, readouts of this assay, unlike those of the [H-3] hypoxanthine incorporation assay, for the antimalarial activity of the above DNA binding agents may be erroneous. In the case of CPPs, false readouts can be improved by the removal of excess peptides.