Identification of SIV env-specific CTL in the jejunal mucosa in vaginally exposed, seronegative rhesus macaques (Macaca mulatta).
Identification of SIV env-specific CTL in the jejunal mucosa in vaginally exposed, seronegative rhesus macaques (Macaca mulatta).
复制标题
阴道暴露、血清阴性恒河猴 (Macaca mulatta) 空肠粘膜中 SIV env 特异性 CTL 的鉴定。
DOI：
10.1034/j.1600-0684.2000.290311.x
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发表时间：
2000
影响因子：
0.7
通讯作者：
Bohm,RP
中科院分区：
文献类型：
--
作者：
Wilson,LA;Murphey-Corb,M;Martin,LN;Harrison,RM;Ratterree,MS;Bohm,RP
我们先前报道了猴结肠暴露于亚感染SIV剂量后空肠固有层（LP）中的主要组织相容性复合物I类限制性细胞毒性T淋巴细胞（CTL）。那些对猿猴免疫缺陷病毒（SIV）包膜（env）具有特异性的强烈粘膜CTL反应的猴子受到保护，免受后来异源病原性病毒剂量的结肠攻击。在此，在阴道暴露于SIV但未感染的8只未经孕酮处理的猕猴中，有5只在空肠LP中类似地诱导了env特异性CTL。孕激素治疗后的阴道激发产生全身感染。仅有的两只攻毒后可检测到空肠env特异性CTL的猴血浆病毒载量显著降低，并延迟了疾病进展。无论是阴道或结肠暴露于亚感染性SIV剂量可以诱导CTL检测空肠LP。这种CTL与攻击后的保护或延迟疾病的关联表明，成功的疫苗保护SIV/HIV可能需要粘膜中的CTL应答。
We previously reported major histocompatibility complex Class I‐restricted cytotoxic T lymphocytes (CTL) in jejunal lamina propria (LP) of monkeys following colonic exposure to subinfectious SIV doses. Those monkeys with strong mucosal CTL responses specific for simian immunodeficiency virus (SIV) envelope (env) were protected from later colonic challenge with a heterologous pathogenic virus dose. Here, env‐specific CTL were similarly induced in jejunal LP in five of eight non‐progesterone treated macaques that were vaginally exposed to SIV, but not infected. Subsequent vaginal challenge following progesterone treatment produced systemic infection. The only two monkeys that had jejunal env‐specific CTL detectable post‐challenge developed significantly lower plasma virus loads, and had delayed disease progression. Either vaginal or colonic exposure to subinfectious SIV doses can induce CTL detectable in jejunal LP. The association of such CTL with protection or delayed disease upon challenge suggests that successful vaccine protection against SIV/HIV may require CTL responses in the mucosa.