Reproducible sampling regimen for specific cortical regions: Application to speech-associated areas
Reproducible sampling regimen for specific cortical regions: Application to speech-associated areas
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DOI：
10.1016/s0165-0270(96)00005-2
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发表时间：
1996-07-01
影响因子：
3
通讯作者：
Kril, JJ
中科院分区：
文献类型：
--
作者：
Harasty, J;Halliday, GM;Kril, JJ
人类大脑外部脑回模式的可变性使得识别特定的皮层区域变得困难。与皮质结构和功能相关的研究并没有一致地控制这种变异性。本研究的目的是开发一种可靠的和可重复的方案，在人脑中的五个语音相关和一个非语音相关的皮层区域的采样。感兴趣的脑回使用非水性染料标记，然后以3 mm的间隔对脑进行冠状切片。使用标记的脑回，建立一组内部脑标志，以帮助采样每个感兴趣的皮质区域的一个块。每个内部标志的位置被确定阿萨大脑总长度和宽度的百分比。使用方差分析研究了每个内部标志位置的变异性，发现在所有情况下在三维空间中一致。在不同情况下，采样皮质区域与内部标志的相关性是一致的，点对点一致性为100%。这与外部脑回特征病例之间的变异性形成对比。通过测量每个皮质层的深度，对取样区域进行测试以确定细胞结构的变异性。该技术发现，在每种情况下都对相同的细胞结构区域进行了采样。正如预期的那样，这些区域可以通过不同皮质层深度的显著差异来区分。外部脑回和内部标志的准确识别发生在90- 100%的评价者之间的点到点一致性。
The variability in the external gyri pattern of human brains has made the identification of specific cortical areas difficult. Studies correlating cortical structure and function have not consistently controlled for this variability. The aim of the present study was to develop a reliable and reproducible regimen for sampling five speech-associated and one non-speech associated cortical region in the human brain. The gyri of interest were labelled using non-aqueous dye prior to coronal slicing of brains at 3 mm intervals. Using the labelled gyri, a set of internal brain landmarks was established to aid in sampling one block of each cortical region of interest. The position of each internal landmark was determined asa percentage of the total brain length and breadth. The variability in the position of each internal landmark was investigated using analysis of variance and found to be consistent in three dimensions in all cases. The correlation of the sampled cortical region to the internal landmark was consistent in different cases with point to point agreement of 100%. This contrasts with the variability between cases in external gyri features. The sampled region was tested to determine cytoarchitectural variability by measuring the depth of each cortical layer. This technique found that the same cytoarchitectural regions were sampled in each case. As expected, these regions were distinguishable by the significant difference in the depth of different cortical layers. Accurate identification of both the external gyri and internal landmarks occurred with interrater point to point agreement of 90-100%.