Effect of edaravone, a novel free radical scavenger, supplemented to cardioplegia on myocardial function after cardioplegic arrest: in vitro study of isolated rat heart
Effect of edaravone, a novel free radical scavenger, supplemented to cardioplegia on myocardial function after cardioplegic arrest: in vitro study of isolated rat heart
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新型自由基清除剂依达拉奉补充心脏停搏液对心脏停搏后心肌功能的影响：离体大鼠心脏的体外研究
DOI：
10.1007/s00380-008-1106-1
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发表时间：
2009
影响因子：
1.5
通讯作者：
M. Komeda
中科院分区：
文献类型：
--
作者：
K. Yamazaki;S. Miwa;S. Toyokuni;S. Nemoto;W. Oriyanhan;K. Takaba;Y. Saji;A. Marui;T. Nishina;T. Ikeda;M. Komeda
心脏停搏是心内直视手术中心肌保护的主要机制，但在缺血再灌注过程中，心脏停搏会引起心肌损伤。众所周知，自由基清除剂可在各种情况下减轻缺血-再灌注损伤。我们研究了依达拉奉（一种最初用于脑保护的新型自由基清除剂）对心脏停搏后缺血再灌注期间心肌功能的影响。切除大鼠心脏，并使用Langendorff装置灌注。每45 min在4°C下使用St.托马斯医院心脏停搏液（ST溶液）心脏停搏90 min，然后再灌注20 min。将心脏分为4组（每组n = 13）。ST组仅用ST液停搏。在L、M和H组中，分别使用补充有低剂量（1 μM）、中等剂量（10 μM）和高剂量（100 μM）依达拉奉的ST溶液使心脏停搏。测定停搏前后左室功能（+dp/dtmax）和心肌酶释放水平。实验结束时，测定心脏含水量和组织氧化应激（8-羟基-2 ′-脱氧鸟苷）。再灌注期间，依达拉奉治疗组的+dp/dtmax表现出更大的功能恢复（P < 0.05）。M组乳酸水平最低（P < 0.01）。依达拉奉组心肌含水量显著低于ST组（P < 0.05），氧化应激显著低于ST组（P < 0.01），以M组最低。在心脏停搏液中加入依达拉奉可通过降低心脏停搏后的氧化应激来改善心肌功能损伤。在本研究中，通过添加中等剂量（10 μM）的依达拉奉实现了心肌功能的最大改善。
Cardioplegic arrest has been the main mechanism of myocardial protection during open-heart surgery; however, it causes myocardial injury during ischemia-reperfusion. Free radical scavengers are widely known to attenuate ischemia-reperfusion injury in various settings. We investigated the effects of edaravone, a novel free radical scavenger that was originally used for cerebral protection, on myocardial function during ischemia-reperfusion after cardioplegic arrest. Rat hearts were excised and perfused using Langendorff apparatus. The hearts were cardioplegically arrested for 90 min using St. Thomas’ Hospital cardioplegic solution (ST solution) at 4°C every 45 min and then reperfused for 20 min. The hearts were divided into 4 groups (n = 13 in each group). In Group ST, the hearts were arrested using the ST solution alone. In Groups L, M, and H, the hearts were arrested using the ST solution supplemented with a low-dose (1 μM), moderate dose (10 μM), and high dose (100 μM) of edaravone, respectively. Left ventricular function (+dp/dtmax) and the levels of the cardiac enzymes released were measured before and after cardioplegic arrest. At the end of the study, the water content and the tissue oxidative stress (8-hydroxy-2′-deoxyguanosine) of the heart were measured. During reperfusion, the edaravone-treated groups showed a greater functional recovery with regard to the +dp/dtmax (P < 0.05). The lactate level was the lowest (P < 0.01) in Group M. The water content of the hearts in the edaravone-treated groups was significantly lower (P < 0.05) than that in Group ST. Oxidative stress was significantly lower (P < 0.01) in the edaravone-treated hearts than in Group ST, and it was the lowest in Group M. The addition of edaravone to the cardioplegic solution ameliorates the impairment in myocardial function by reducing the oxidative stress after cardioplegic arrest. In this study, the maximum improvement in the myocardial function was achieved by addition of a moderate dose (10 μM) of edaravone.
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影响因子：
5
作者：
VandenHoek, TL;Li, CQ;Becker, LB
通讯作者：
Becker, LB
影响因子：
20.1
作者：
Vanden Hoek, TL;Becker, LB;Schumacker, PT
通讯作者：
Schumacker, PT
影响因子：
20.1
作者：
LAINE, GA;ALLEN, SJ
通讯作者：
ALLEN, SJ
心肌水肿对左心室压力-容积关系的定量影响。
DOI：
--
发表时间：
1993
期刊：
The Journal of thoracic and cardiovascular surgery
影响因子：
--
作者：
Hsu,DT;Weng,ZC;Nicolosi,AC;Detwiler,PW;Sciacca,R;Spotnitz,HM
通讯作者：
Spotnitz,HM
反对超氧化物歧化酶治疗实验性心肌梗死的“早期保护延迟死亡”假说的证据。
影响因子：
20.1
作者：
Tanaka,M;Stoler,RC;FitzHarris,GP;Jennings,RB;Reimer,KA
通讯作者：
Reimer,KA