Molecular Interactions of Protein with TiO2 by the AFM-Measured Adhesion Force
Molecular Interactions of Protein with TiO2 by the AFM-Measured Adhesion Force
复制标题
通过 AFM 测量的粘附力观察蛋白质与 TiO2 的分子相互作用
DOI：
10.1021/acs.langmuir.7b02024
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发表时间：
2017-10-24
期刊：
影响因子：
3.9
通讯作者：
Lu, Xiaohua
中科院分区：
文献类型：
--
作者：
Dong, Yihui;An, Rong;Lu, Xiaohua
了解多孔材料和生物系统之间的相互作用对于生物医学和环境科学非常重要。根据原子力显微镜 (AFM) 粘附力测量，本文提出了一种新的实验方法来确定蛋白质和各种表面粗糙度的介孔 TiO2 之间的分子相互作用力。通过拟合单位接触面积的粘附力和吸附能力来表征每个蛋白质分子与纯锐钛矿型 TiO2 表面之间的相互作用力，发现溶菌酶、肌红蛋白和 BSA 的粘附力分别约为 0.86、2.63 和 4.41 nN。此外，我们报道分子相互作用力与材料的表面形貌无关，但蛋白质类型是相互作用的一个因素。这些分子水平的实验结果为刺激蛋白质与表面相互作用的模型计算和分子模拟研究提供了有用的见解。
Understanding the interactions between porous materials and biosystems is of great important in biomedical and environmental sciences. Upon atomic force microscopy (AFM) adhesion measurement, a new experimental approach was presented here to determine the molecular interaction force between proteins and mesoporous TiO2 of various surface roughnesses. The interaction force between each protein molecule and the pure anatase TiO2 surface was characterized by fitting the adhesion and adsorption capacity per unit contact area, and it was found that the adhesion forces were approximately 0.86, 2.63, and 4.41 nN for lysozyme, myoglobin, and BSA, respectively. Moreover, we reported that the molecular interaction force was independent of the surface topography of the material but the protein type is a factor of the interaction. These experimental results on the molecular level provide helpful insights for stimulating model calculation and molecular simulation studies of protein interaction with surfaces.