One- and two-photon induced fluorescence of pacific blue-labeled human serum albumin deposited on different core size silver colloids
One- and two-photon induced fluorescence of pacific blue-labeled human serum albumin deposited on different core size silver colloids
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DOI：
10.1002/bip.20407
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发表时间：
2006-03-01
期刊：
影响因子：
2.9
通讯作者：
Gryczynski, Zygmunt
中科院分区：
文献类型：
--
作者：
Lukomska, Joanna;Gryczynski, Ignacy;Gryczynski, Zygmunt
研究了在不同尺寸银胶存在下，太平洋蓝(PB)标记的人血清白蛋白(HSA)的单光子和双光子诱导荧光。观察到了小胶体(30-40 nm)和大胶体(约120 nm)的PB荧光发射强度，并与没有胶体的PB发射进行了比较。对于核尺寸较小的胶体体系，单光子激发没有检测到任何荧光增强，而双光子激发观察到的荧光增强约为2.5倍。相比之下，对于大尺寸的银胶，我们观察到单光子激发的PB荧光亮度增加了约2倍，而双光子激发的增强作用增加了13倍。与单光子激发相比，双光子激发的亮度增加更大，表明增强的局域场在溶液中银胶的荧光增强中起主导作用。(C)2005年威利期刊公司。
We studied one- and two-photon induced fluorescence of Pacific Blue (PB)-labeled human serum albumin (HSA) in the presence of different size silver colloids. The PB fluorescence emission intensity was observed with small (30-40 nm) and large (about 120 nm) colloids and compared with PB emission in absence of colloids. For the system with a small core size colloids we did not detect any fluorescence enhancement with one-photon excitation and the enhancement observed with two-photon excitation was about 2.5-fold. In contrast, for large silver colloids we observed about a 2-fold increase in PB fluorescence brightness for one-photon excitation, and the enhancement with two-photon excitation excided 13-folds. Much stronger increases in brightness observed with two-photon excitation, compared to one-photon excitation, indicate a dominant role of enhanced local field in fluorescence enhancement on silver colloids in solutions. (c) 2005 Wiley Periodicals, Inc.