Tip size of ion-exchanger based K+-selective microelectrodes. II. Effects on measurement of evoked [K+]0 transients.
Tip size of ion-exchanger based K+-selective microelectrodes. II. Effects on measurement of evoked [K+]0 transients.
复制标题
基于离子交换剂的 K 选择性微电极的尖端尺寸。
DOI：
10.1139/y87-144
复制
发表时间：
1987
影响因子：
2.1
通讯作者：
Yamate,CL
中科院分区：
文献类型：
--
作者：
Ransom,BR;Carlini,WG;Yamate,CL
以双管离子交换器为基础的K+选择性微电极(K+ISM)用于预测微电极尖端引起的组织损伤对测量的[K+]0升高幅度的影响。大鼠视神经刺激引起的[K+]0升高的幅度取决于测量K+ISM的尖端直径；较小的倾斜K+ISM往往比较大的倾斜K+ISM测量更大的[K+]0升高。当用2个不同尖端大小的K+ISM同时记录刺激[K+]0时，小尖端K+ISM记录的[K+]0增加，平均比大尖端K+ISM测量的增加大14%。鉴于这些结果，以及前一篇论文中提出的结果，研究人员应该标准化实验中使用的ISM的尖端大小。
Double-barreled ion-exchanger based K+-selective microelectrodes (K+ISMs) of a variety of tip diameters were used to provide an experimental test of predictions that microelectrode tip induced tissue damage influences the magnitude of measured [K+]0increases. The measured magnitude of stimulation induced [K+]0rises in the rat optic nerve depended on the tip diameter of the measuring K+ISM; smaller tipped K+ISMs tended to measure larger rises of [K+]0than bigger tipped K+ISMs. When stimulated [K+]0increases were simultaneously recorded with 2 K+ISMs of different tip size, the smaller tipped K+ISM recorded [K+]0increases that were, on average, 14% larger than the increases measured by the bigger tipped K+ISM. In view of these results, and those presented in the previous paper, investigators should standardize the tip sizes of the ISMs used in their experiments.