Transcutaneous DNA immunization following waxing-based hair depilation.
Transcutaneous DNA immunization following waxing-based hair depilation.
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打蜡脱毛后进行经皮 DNA 免疫。
DOI：
10.1016/j.jconrel.2011.08.038
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Cui,Zhengrong
中科院分区：
文献类型：
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作者：
Sloat,BrianR;Kiguchi,Kaoru;Xiao,Gang;DiGiovanni,John;Maury,Wendy;Cui,Zhengrong
经皮DNA免疫是一种有吸引力的免疫方法。先前，我们报道了通过将质粒DNA应用于皮肤区域的经皮免疫，其中毛囊已经通过基于“冷”上蜡的毛发拔除被诱导进入生长阶段，显著增强了所得的免疫应答。在本研究中，使用编码炭疽芽孢杆菌保护性抗原（PA 63）基因片段的质粒，显示通过将质粒施加到通过“温”上蜡拔毛的皮肤区域上诱导的抗PA 63抗体应答显著强于通过“冷”上蜡拔毛，这很可能是因为“温暖的”蜡基脱毛显著i）增强了吸收在一些实施方案中，所述方法包括：i）在应用区域中质粒的表达（或保留）;和ii）增强转染基因在皮肤的毛囊和毛囊间表皮中的表达。经皮DNA免疫诱导的抗体反应是毛发周期依赖性的，因为质粒需要在拔毛后5d内应用以诱导强烈的抗体反应。抗体应答不受表达的PA 63蛋白作为抗原是否分泌或细胞表面结合的影响。最后，这种增强在“温”蜡脱毛后通过经皮DNA免疫诱导的免疫应答的策略并不限于作为抗原的PA 63，因为用编码HIV-1 env gp 160基因的质粒免疫也诱导了强烈的抗gp 160应答。通过改变毛囊周期的经皮DNA免疫可能在诱导强烈的功能性免疫应答方面具有很大的前景。
Transcutaneous DNA immunization is an attractive immunization approach. Previously, we reported that transcutaneous immunization by applying plasmid DNA onto a skin area wherein the hair follicles had been induced into growth stage by ‘cold’ waxing-based hair plucking significantly enhanced the resultant immune responses. In the present study, using a plasmid that encodes the Bacillus anthracis protective antigen (PA63) gene fragment, it was shown that the anti-PA63 antibody responses induced by applying the plasmid onto a skin area where the hair was plucked by ‘warm’ waxing were significantly stronger than by ‘cold’ waxing, very likely because the ‘warm’ waxing-based hair depilation significantly i) enhanced the uptake (or retention) of the plasmid in the application area and ii) enhanced the expression of the transfected gene in the follicular and interfollicular epidermis in the skin. The antibody response induced by transcutaneous DNA immunization was hair cycle dependent, because the plasmid needed to be applied within 5days after the hair plucking to induce a strong antibody response. The antibody responses were not affected by whether the expressed PA63 protein, as an antigen, was secreted or cell surface bound. Finally, this strategy of enhancing the immune responses induced by transcutaneous DNA immunization following ‘warm’ waxing-based hair depilation was not limited to the PA63 as an antigen, because immunization with a plasmid that encodes the HIV-1 env gp160 gene induced a strong anti-gp160 response as well. Transcutaneous DNA immunization by modifying the hair follicle cycle may hold a great promise in inducing strong and functional immune responses.